Enzymatic hydrolysis of sodium dodecyl sulphate (SDS)-pretreated newspaper for cellulosic ethanol production by Saccharomyces cerevisiae and Pichia stipitis

Applied Biochemistry and Biotechnology
Fengxue XinMing Jun Marcus Gum

Abstract

Fermentation of enzymatic hydrolysate of waste newspaper was investigated for cellulosic ethanol production in this study. Various nonionic and ionic surfactants were applied for waste newspaper pretreatment to increase the enzymatic digestibility. The surfactant-pretreated newspaper was enzymatically digested in 0.05 M sodium citrate buffer (pH 4.8) with varying solid content, filter paper unit loading (FPU/g newspaper), and ratio of filter paper unit/beta-glucosidase unit (FPU/CBU). Newspaper pretreated with the anionic surfactant sodium dodecyl sulphate (SDS) demonstrated the highest sugar yield. The addition of Tween-80 in the enzymatic hydrolysis process enhanced the enzymatic digestibility of newspaper pretreated with all of the surfactants. Enzymatic hydrolysis of SDS-pretreated newspaper with 15% solid content, 15 FPU/g newspaper, and FPU/CBU of 1:4 resulted in a newspaper hydrolysate conditioning 29.07 g/L glucose and 4.08 g/L xylose after 72 h of incubation at 50 degrees C. The fermentation of the enzymatic hydrolysate with Saccharomyces cerevisiae, Pichia stipitis, and their co-culture produced 14.29, 13.45, and 14.03 g/L of ethanol, respectively. Their corresponding ethanol yields were 0.43, 0.41, and 0.42 g/g.

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Citations

Jul 2, 2014·Bioresource Technology·Fang-Chen WuIng-Lung Shih
Mar 5, 2016·AMB Express·Salvatore MontellaVincenza Faraco
Nov 22, 2011·Bioresource Technology·Anli GengJun-yu Ip
Aug 12, 2014·Applied Biochemistry and Biotechnology·Vladimir BrummerPetr Stehlik
Sep 21, 2011·Critical Reviews in Biotechnology·Anuj K ChandelSilvio Silvério da Silva
Apr 1, 2014·Preparative Biochemistry & Biotechnology·Akarin BoonsombutiSujitra Wongkasemjit
Mar 15, 2014·Applied Biochemistry and Biotechnology·Luana Parras MeleiroRosa Prazeres Melo Furriel
Feb 24, 2016·Tropical Animal Health and Production·Thiwakorn AmpaponSungchhang Kang

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