PMID: 60178Feb 1, 1976Paper

Enzymatic in vitro synthesis of globin genes

Cell
A EfstratiadisT Maniatis

Abstract

Full-length, single-stranded rabbit globin cDNA, synthesized by AMV reverse transcriptase, apparently contains a small double-stranded sequence (hairpin) at the 3' terminus. This cDNA can serve as template-primer for E. coli DNA polymerase I, which synthesizes a strand complementary to the cDNA and covalently bound to it. The loop connecting the two strands can be cut by S1 nuclease. Reassociation, hybridization, and restriction endonuclease studies, as well as electrophoretic analyses, indicate that the sequential actions of reverse transcriptase, DNA polymerase 1, and S1 nuclease generate full-length, double-stranded synthetic globin genes.

References

Aug 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J N VournakisF C Kafatos
Jan 1, 1975·Nucleic Acids Research·K OlsonC Harvey
Jun 1, 1975·European Journal of Biochemistry·D J Arndt-JovinM Marquardt
Nov 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·K FujinagaM Green
Feb 9, 1972·Nature: New Biology·A BankP A Marks
Aug 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·J P Leis, J Hurwitz
Jul 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·J G Stavrianopoulos, E Chargaff
Nov 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·A E SippelP Feigelson
Dec 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·W Gilbert, A Maxam

❮ Previous
Next ❯

Citations

Feb 8, 2007·Analytical and Bioanalytical Chemistry·Akihiro SawadaYoshinobu Baba
Oct 1, 1983·Developmental Biology·T R Tansey, J V Ruderman
Apr 20, 1987·Journal of Molecular Biology·S J Smith-GillB R Brooks
Sep 20, 1988·Journal of Molecular Biology·N H Brown, F C Kafatos
Apr 1, 1987·Molecular and Biochemical Parasitology·S HeathJ M Crampton
Jun 24, 1985·Biochimica Et Biophysica Acta·J W Gaubatz, G V Paddock
Mar 1, 1986·Veterinary Parasitology·H R Gamble, D S Zarlenga
Apr 28, 2000·Journal of Biotechnology·F Sterky, J Lundeberg
Sep 18, 2013·Proceedings of the National Academy of Sciences of the United States of America·Stanley N Cohen
Oct 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·F Rougeon, B Mach
Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·R M Lawn
Oct 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·B M PatersonE L Kuff
Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M BouloyR M Krug
Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M E SobelI Pastan
Aug 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·L Villa-KomaroffW Gilbert
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H M KronenbergA Rich
Oct 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·S J ChanD F Steiner
Dec 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·B J SchmeckpeperC C Talbot
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·P GrussG Khoury

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell eTOC

Cell is a scientific journal publishing research across a broad range of disciplines within the life sciences field. Discover the latest research from Cell here.

CZI Human Cell Atlas Seed Network

The aim of the Human Cell Atlas (HCA) is to build reference maps of all human cells in order to enhance our understanding of health and disease. The Seed Networks for the HCA project aims to bring together collaborators with different areas of expertise in order to facilitate the development of the HCA. Find the latest research from members of the HCA Seed Networks here.