Jul 1, 1977

Enzymatic isolation of osteoblasts from fetal rat calvaria

The Anatomical Record
J A Yagiela, D M Woodbury


A microanatomical study of the immature rat calvarium was performed in order to develop a method for the isolation of osteoblasts by enzymatic means. Although generalized osteogenesis was evident in fetal rats, differential growth patterns were observed beginning at 19 days in utero. Considerable portions of the endocranial periosteal bone surface were lined by flattened, less active cells; discrete areas also contained multinucleated osteoclasts. Cell counts of whole calvaria revealed that one fifth of the total cell population were osteoblasts, most of which were located in the central portions of the frontal and parietal bones. Prior excision of these segments permitted the subsequent removal of virtually all periosteal tissues. Cleaned 19-day fetal bones, incubated in crude collagenase for two hours, released about 40,000 cells/calvarium, consisting of 85-90% osteoblasts and lesser amounts of connective tissue and bone marrow elements. Because of the relatively small sizes of most extraneous cells, purity on a cell volume basis was approximately 95%. It is predicted this preparation will be useful in the investigation of certain aspects of osteoblastic function.

Mentioned in this Paper

Developmental Growth Involved in Morphogenesis
Bone Marrow
Cell Count
Gestational Age
Bone Growth
Fetal Bones

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Bone Marrow Neoplasms

Bone Marrow Neoplasms are cancers that occur in the bone marrow. Discover the latest research on Bone Marrow Neoplasms here.