Enzymatic Mechanisms Involved in Phenanthrene Degradation by the White Rot Fungus Pleurotus ostreatus.

Applied and Environmental Microbiology
L BezalelC E Cerniglia

Abstract

The enzymatic mechanisms involved in the degradation of phenanthrene by the white rot fungus Pleurotus ostreatus were examined. Phase I metabolism (cytochrome P-450 monooxygenase and epoxide hydrolase) and phase II conjugation (glutathione S-transferase, aryl sulfotransferase, UDP-glucuronosyltransferase, and UDP-glucosyltransferase) enzyme activities were determined for mycelial extracts of P. ostreatus. Cytochrome P-450 was detected in both cytosolic and microsomal fractions at 0.16 and 0.38 nmol min(sup-1) mg of protein(sup1), respectively. Both fractions oxidized [9,10-(sup14)C]phenanthrene to phenanthrene trans-9,10-dihydrodiol. The cytochrome P-450 inhibitors 1-aminobenzotriazole (0.1 mM), SKF-525A (proadifen, 0.1 mM), and carbon monoxide inhibited the cytosolic and microsomal P-450s differently. Cytosolic and microsomal epoxide hydrolase activities, with phenanthrene 9,10-oxide as the substrate, were similar, with specific activities of 0.50 and 0.41 nmol min(sup-1) mg of protein(sup-1), respectively. The epoxide hydrolase inhibitor cyclohexene oxide (5 mM) significantly inhibited the formation of phenanthrene trans-9,10-dihydrodiol in both fractions. The phase II enzyme 1-chloro-2,4-dinitrobenzene glutathione S-transfer...Continue Reading

References

Jan 1, 1978·Methods in Enzymology·A G HildebrandtG Heinemeyer
Jan 1, 1992·Journal of Industrial Microbiology·J B Sutherland
Jun 1, 1992·Applied and Environmental Microbiology·K E HammelM A Moen
Dec 18, 1991·European Journal of Biochemistry·M H JacobsD B Janssen
Nov 1, 1991·Applied and Environmental Microbiology·J B SutherlandC E Cerniglia
Jan 1, 1991·Advances in Applied Microbiology·F S Sariaslani
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·K E HammelW Z Gai
Jan 1, 1990·Methods in Enzymology·J W WhittakerJ D Lipscomb
May 1, 1990·Applied and Environmental Microbiology·S HartmansJ A de Bont
Jan 1, 1989·Applied and Environmental Microbiology·J A Bumpus
Jan 1, 1982·Methods in Enzymology·P Gierow, B Jergil
Jul 15, 1983·Archives of Biochemistry and Biophysics·D E Matthews, H D Van Etten
Mar 1, 1995·Applied Microbiology and Biotechnology·C D BarclayR L Legge
Jul 1, 1996·Applied and Environmental Microbiology·L BezalelC E Cerniglia
Jan 1, 1996·Applied and Environmental Microbiology·L BezalelC E Cerniglia

❮ Previous
Next ❯

Citations

Dec 1, 2000·Applied and Environmental Microbiology·L M LarrayaL Ramírez
Jan 15, 2008·Applied Microbiology and Biotechnology·Kyung MyungJan A Narciso
Jan 18, 2012·Applied Microbiology and Biotechnology·Ilaria CiulliniFabrizio Briganti
Jan 3, 2013·Microbial Biotechnology·Mélanie MorelEric Gelhaye
May 8, 2013·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Chang-Young HongIn-Gyu Choi
Nov 23, 2016·Journal of Basic Microbiology·Sourav BhattacharyaJayaraman Angayarkanni
Jan 20, 2017·Current Microbiology·Adi Setyo PurnomoIchiro Kamei
Feb 2, 2000·Applied and Environmental Microbiology·C Johannes, A Majcherczyk
Feb 24, 2006·Journal of Industrial Microbiology & Biotechnology·I EichlerováF Nerud
Nov 15, 2007·Journal of Environmental Science and Health. Part C, Environmental Carcinogenesis & Ecotoxicology Reviews·Huey-Min HwangXueheng Zhao
Nov 6, 2013·Applied Microbiology and Biotechnology·Kateřina SvobodováDenisa Petráčková
Apr 24, 2013·FEMS Microbiology Letters·Carlos E Rodríguez-RodríguezKarla Ruiz-Hidalgo
Jun 24, 2020·Applied Microbiology and Biotechnology·Hongjae Park, In-Geol Choi
Sep 2, 2010·Journal of Basic Microbiology·Rathinasamy Periasamy, Thayumanavan Palvannan
Oct 20, 2010·Archives of Environmental Contamination and Toxicology·Catarina MoreirinhaFernanda Cássio
Jul 1, 2015·Folia Microbiologica·Denisa PetráčkováKateřina Svobodová
Apr 17, 2013·Biotechnology and Applied Biochemistry·Hirofumi Ichinose
Feb 7, 2003·Applied and Environmental Microbiology·Jonathan D Van HammeMichael A Pickard
Feb 6, 2009·Current Microbiology·Svetlana V NikiforovaOlga V Turkovskaya
Aug 12, 2014·Applied Biochemistry and Biotechnology·Zulfiye Velioglu, Raziye Ozturk Urek
Feb 21, 2014·Bioprocess and Biosystems Engineering·Tony Hadibarata, Zee Chuang Teh
Oct 30, 2014·Environmental Technology·Antonella GullottoFabrizio Briganti
Sep 22, 2016·Bioscience, Biotechnology, and Biochemistry·Masahito SuikoMing-Cheh Liu
Mar 7, 2019·Extremophiles : Life Under Extreme Conditions·Martina HujslováMilan Gryndler
May 29, 2010·Applied Microbiology and Biotechnology·Nomathemba Loice ChiguHiroyuki Wariishi
Sep 3, 1999·Applied and Environmental Microbiology·M A PickardR Vazquez-Duhalt
Apr 11, 2019·PeerJ·Alona S SekanYaroslav Blume
Dec 3, 1999·Applied and Environmental Microbiology·H VisserJ C Verdoes
Jul 31, 1999·Applied and Environmental Microbiology·L M LarrayaL Ramírez
Nov 24, 2019·International Journal of Environmental Research and Public Health·Laura N LevinGuadalupe Gutiérrez-Soto
Aug 16, 2018·World Journal of Microbiology & Biotechnology·Natalia PozdnyakovaOlga Turkovskaya
Feb 28, 2019·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Natalia N PozdnyakovaOlga V Turkovskaya
May 24, 2020·Environmental Pollution·Enrica PicarielloFlavia De Nicola

❮ Previous
Next ❯

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.