Enzymatic modification of daidzin using heterologously expressed amylosucrase in Bacillus subtilis

Food Science and Biotechnology
Eun-Ryoung KimCheon-Seok Park

Abstract

Amylosucrases (ASase, EC 2.4.1.4) from Deinococcus geothermalis (DGAS) and Neisseria polysaccharea (NPAS) were heterologously expressed in Bacillus subtilis. While DGAS was successfully expressed, NPAS was not. Instead, NPAS was expressed in Escherichia coli. Recombinant DGAS and NPAS were purified using nickel-charged affinity chromatography and employed to modify daidzin to enhance its water solubility and bioavailability. Analyses by LC/MS revealed that the major products of transglycosylation using DGAS were daidzein diglucoside and daidzein triglucoside, whereas that obtained by NPAS was only daidzein diglucoside. The optimal bioconversion conditions for daidzein triglucoside, which was predicted to have the highest water-solubility among the daidzin derivatives, was determined to be 4% (w/v) sucrose and 250 mg/L daidzin in sodium phosphate pH 7.0, with a reaction time of 12 h. Taken together, we suggest that the yield and product specificity of isoflavone daidzin transglycosylation may be modulated by the source of ASase and reaction conditions.

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Citations

Jan 25, 2020·Food Science and Biotechnology·Dong-Ho SeoCheon-Seok Park
Jan 8, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Young Sung JungDae-Ok Kim
Dec 29, 2020·Current Opinion in Chemical Biology·Claire MoulisMagali Remaud-Siméon

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