Enzymatic oxidation of tetramethyl-p-phenylenediamine and p-phenylenediamine by the electron transport particulate fraction of Azotobacter vinelandii.

Journal of Bacteriology
P JurtshukL Old

Abstract

The ability of the electron transport particulate fraction of Azotobacter vinelandii strain O to oxidize tetramethyl-p-phenylenediamine (TMPD) and p-phenylenediamine (PPD) was examined in detail. The highest specific activity for TMPD and PPD oxidation concentrated in the A. vinelandii O R(3) fraction. The A. vinelandii O R(3) fraction was used to develop a standard manometric assay which gave optimal oxidation rates for both of these dyes. The conditions of the assay and all essential related enzymatic kinetic parameters are presented. Other para derivatives of phenylenediamines also were oxidized readily, whereas ortho and meta derivatives were not. Hydroquinone, p-hydroxybenzoic acid, p-cresol, tyrosine, pyrogallol, pyrocatechol, and diphenylamine were not able to serve as electron donors for the A. vinelandii O R(3) system. The probable involvement of a particle-bound cytochrome oxidase is indicated by the marked sensitivity of both TMPD and PPD oxidation to cyanide, axide, phenylhydrazine, hydroxylamine, and, to a lesser degree, carbon monoxide.

Citations

Dec 1, 1975·FEBS Letters·A J Downs, C W Jones
Dec 1, 1984·Journal of Bioenergetics and Biomembranes·T Y Wong, P Jurtshuk
Jan 1, 1978·CRC Critical Reviews in Microbiology·S A Morse
May 1, 1975·CRC Critical Reviews in Microbiology·P JurtshukW C Acord
May 11, 1971·European Journal of Biochemistry·B A Ackrell, C W Jones
Jan 1, 1982·European Journal of Biochemistry·T Yang
Jan 1, 1990·Membrane Biochemistry·J C Cox, P Jurtshuk
Jan 4, 2019·International Journal of Systematic and Evolutionary Microbiology·Guang PengLi-Xing Zhao
Dec 16, 1970·Biochimica Et Biophysica Acta·C R Barrera, P Jurtshuk
Sep 14, 1981·Biochimica Et Biophysica Acta·P JurtshukT Y Wong
Nov 19, 1974·Biochimica Et Biophysica Acta·P Jurtshuk, L McManus
Feb 22, 1974·Biochimica Et Biophysica Acta·H F Kauffman, B F Van Gelder
Aug 5, 1969·Biochimica Et Biophysica Acta·R T Swank, R H Burris
Jun 8, 1978·Biochimica Et Biophysica Acta·T Y Yang, P Jurtshuk
May 11, 1976·Applied and Environmental Microbiology·P Jurtshuk, D N McQuitty
Feb 1, 1986·Infection and Immunity·F S Archibald, M N Duong
Dec 1, 1975·Applied Microbiology·P JurtshukD N McQuitty
Mar 1, 1969·Journal of Bacteriology·P Jurtshuk, B A Schlech
Apr 14, 1978·Biochemical and Biophysical Research Communications·T Y Yang, P Jurtshuk

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.