Enzymatic polymerization of dihydroquercetin using bilirubin oxidase

Biochemistry. Biokhimii︠a︡
M E KhlupovaA I Yaropolov

Abstract

Dihydroquercetin (or taxifolin) is one of the most famous flavonoids and is abundant in Siberian larch (Larix sibirica). The oxidative polymerization of dihydroquercetin (DHQ) using bilirubin oxidase as a biocatalyst was investigated and some physicochemical properties of the products were studied. DHQ oligomers (oligoDHQ) with molecular mass of 2800 and polydispersity of 8.6 were obtained by enzymatic reaction under optimal conditions. The oligomers appeared to be soluble in dimethylsulfoxide, dimethylformamide, and methanol. UV-visible spectra of oligoDHQ in dimethylsulfoxide indicated the presence of highly conjugated bonds. The synthesized oligoDHQ was also characterized by FTIR and (1)H and (13)C NMR spectroscopy. Comparison of NMR spectra of oligoDHQ with DHQ monomer and the parent flavonoids revealed irregular structure of a polymer formed via the enzymatic oxidation of DHQ followed by nonselective radical polymerization. As compared with the monomer, oligoDHQ demonstrated higher thermal stability and high antioxidant activity.

References

Nov 1, 1991·Applied Biochemistry and Biotechnology·J GuoG X Li
Jun 5, 1997·Nature·J JankunE Skrzypczak-Jankun
Jan 1, 1997·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·P C Hollman, M B Katan
Feb 9, 2002·The Journal of Nutritional Biochemistry·Alessandra BordoniPier L. Biagi
Dec 28, 2002·Journal of Natural Products·Rita AquinoLuca Rastrelli
Sep 10, 2003·Biomacromolecules·Motoichi KurisawaShiro Kobayashi
Jun 14, 2006·Biomacromolecules·Rosa Martha Desentis-MendozaMaría Eugenia Jaramillo-Flores
Jul 8, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Kimihiko MizutaniBunzo Mikami
Nov 25, 2011·Enzyme and Microbial Technology·Tukayi KudangaStephanie Burton
May 1, 1998·Journal of Agricultural and Food Chemistry·Ann E HagermanThomas L Riechel

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Citations

Apr 30, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Anastasia ZervaEvangelos Topakas

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