Enzymatic pretreatment of Chlorella vulgaris for biogas production: Influence of urban wastewater as a sole nutrient source on macromolecular profile and biocatalyst efficiency

Bioresource Technology
Ahmed MahdyCristina González-Fernández

Abstract

Two biocatalysts, namely carbohydrases and proteases, were assessed for organic matter solubilisation and methane yield enhancement of microalgae biomass. This study evidenced Chlorella vulgaris carbohydrate accumulation (40% on VSS basis) when grown in urban wastewater. Despite of the carbohydrate prevailing fraction, protease pretreatment showed higher organic matter hydrolysis efficiency (54%). Microscopic observation revealed that carbohydrases affected slightly the cell wall while protease was not selective to wall constituents. Raw and pretreated biomass was digested at 1.5kgtCODm(-3)day(-1) organic loading rate (OLR1) and 20days hydraulic retention time (HRT). The highest methane yield (137mLCH4gCODin(-1)) was achieved in the reactor fed with protease pretreated C. vulgaris. Additionally, anaerobic digestion was conducted at OLR2 (3kgtCODm(-3)day(-1)) and HRT (15days). When compared to raw biomass, methane yield increased 5- and 6.3-fold at OLR1 and OLR2, respectively. No inhibitors were detected during the anaerobic digestion.

References

Aug 4, 2010·Bioresource Technology·Monique RasJean-Philippe Steyer
Mar 16, 2011·Biotechnology and Bioengineering·Irena BrányikováMilada Vítová
Feb 18, 2012·Bioresource Technology·C González-FernándezJ P Steyer
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Jul 24, 2012·Biotechnology Advances·Cristina González-Fernández, Mercedes Ballesteros
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Oct 8, 2013·Bioresource Technology·Lara MendezCristina González-Fernández
Apr 9, 2015·International Journal of Molecular Sciences·Dongqin WangMin Zhong

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Citations

May 8, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jose Antonio MagdalenaCristina González-Fernandez
Mar 12, 2018·Environmental Science and Pollution Research International·Santiago Barreiro-VescovoCristina González-Fernández
Aug 14, 2018·Water Science and Technology : a Journal of the International Association on Water Pollution Research·N ShchegolkovaD Karyakin
Oct 4, 2018·Biotechnology Progress·Jose Antonio MagdalenaCristina González-Fernandez
Dec 8, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jose Antonio Magdalena, Cristina González-Fernández
May 28, 2019·Applied Biochemistry and Biotechnology·Olivia CórdovaRolando Chamy
Nov 20, 2020·The Science of the Total Environment·Seyedeh Fatemeh MohsenpourTony Gutierrez
Sep 6, 2020·The Science of the Total Environment·Shashi Kant BhatiaYung-Hun Yang

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