Enzymatic processing of uracil glycol, a major oxidative product of DNA cytosine.

The Journal of Biological Chemistry
A A PurmalS S Wallace

Abstract

A major stable oxidation product of DNA cytosine is uracil glycol (Ug). Because of the potential of Ug to be a strong premutagenic lesion, it is important to assess whether it is a blocking lesion to DNA polymerase as is its structural counterpart, thymine glycol (Tg), and to evaluate its pairing properties. Here, a series of oligonucleotides containing Ug or Tg were prepared and used as templates for a model enzyme, Escherichia coli DNA polymerase I Klenow fragment (exo-). During translesion DNA synthesis, Ug was bypassed more efficiently than Tg in all sequence contexts examined. Furthermore, only dAMP was incorporated opposite template Ug and Tg and the kinetic parameters of incorporation showed that dAMP was inserted opposite Ug more efficiently than opposite Tg. Ug opposite G and A was also recognized and removed in vitro by the E. coli DNA repair glycosylases, endonuclease III (endo III), endonuclease VIII (endo VIII), and formamidopyrimidine DNA glycosylase. The steady state kinetic parameters indicated that Ug was a better substrate for endo III and formamidopyrimidine DNA glycosylase than Tg; for endonuclease VIII, however, Tg was a better substrate.

References

Apr 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J R WagnerB N Ames
Jan 8, 1991·Biochemistry·T J McBrideL A Loeb
Jan 1, 1990·Free Radical Biology & Medicine·Y Sun
Aug 1, 1988·Journal of Bacteriology·M F Laspia, S S Wallace
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A K BasuJ M Essigmann
Jan 1, 1989·Free Radical Research Communications·B N Ames
Mar 11, 1989·Nucleic Acids Research·R E Dickerson
Apr 1, 1987·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·R Téoule
Aug 25, 1987·Biochemistry·J M Clark, G P Beardsley
Jan 24, 1986·Nucleic Acids Research·R C Hayes, J E LeClerc
Jul 5, 1994·Proceedings of the National Academy of Sciences of the United States of America·D I FeigL A Loeb
Jun 1, 1995·Free Radical Biology & Medicine·A P Breen, J A Murphy
Jan 1, 1994·Annual Review of Biochemistry·B Demple, L Harrison
Jul 29, 1994·Annals of the New York Academy of Sciences·M F GoodmanR Eritja
Jul 29, 1994·Annals of the New York Academy of Sciences·J MillerR Osman
Jun 1, 1993·International Journal of Radiation Biology·K MiaskiewiczR Osman
Sep 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·B N AmesT M Hagen
Apr 4, 1997·The Journal of Biological Chemistry·H C Kung, P H Bolton

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Citations

Oct 29, 2002·International Journal of Cancer. Journal International Du Cancer·Mariko MurataShosuke Kawanishi
Apr 25, 2012·Extremophiles : Life Under Extreme Conditions·Xiaoting HuaYuejin Hua
Nov 26, 2003·Mutation Research·Svein Bjelland, Erling Seeberg
Jun 28, 2002·Free Radical Biology & Medicine·Susan S Wallace
Apr 26, 2003·DNA Repair·Susan S WallaceJeffrey P Bond
Aug 2, 2003·DNA Repair·Dmitry O ZharkovArthur P Grollman
Jun 26, 2012·Chemical Research in Toxicology·Francois Samson-ThibaultJ Richard Wagner
Sep 11, 2010·Chemical Communications : Chem Comm·Joshy Joseph, Gary B Schuster
Sep 26, 2001·The Journal of Biological Chemistry·S TornalettiP C Hanawalt
Mar 17, 2007·Critical Reviews in Food Science and Nutrition·Eun-Sun Hwang, Phyllis E Bowen
Nov 23, 2007·Nucleic Acids Research·Sébastien Tremblay, J Richard Wagner
Jan 7, 2003·Genes & Development·Robert E JohnsonLouise Prakash
Dec 18, 2013·PLoS Pathogens·Liang-Dong LyuGuo-Ping Zhao
Aug 5, 2006·Genetics·Ana I PrietoJosep Casadesús
Jan 12, 2002·Biochemical and Biophysical Research Communications·Shiho Ohnishi, Shosuke Kawanishi
Jul 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Sylvie DoubliéSusan S Wallace
Dec 4, 2004·Mutation Research·Katsuhisa SakanoShosuke Kawanishi
Sep 24, 2004·DNA Repair·Elisabeth LarsenArne Klungland
Sep 25, 2012·Biochimie·Nina G DolinnayaTatiana S Oretskaya
Jan 15, 2014·Critical Reviews in Biochemistry and Molecular Biology·Dawit KidaneJoann B Sweasy
Dec 15, 2007·Biochemical and Biophysical Research Communications·Varatharasa ThiviyanathanDavid G Gorenstein
Feb 2, 2012·Cancer Letters·Miral Dizdaroglu
Feb 20, 1999·Molecular Cell·A KlunglandT Lindahl
Mar 22, 2015·Mutation Research. Reviews in Mutation Research·Miral Dizdaroglu
Jun 24, 2006·Methods in Enzymology·Viswanath BandaruSusan S Wallace
Feb 26, 2000·Carcinogenesis·L J Marnett
Feb 23, 2008·Organic & Biomolecular Chemistry·Avik GhoshJean Cadet
Oct 2, 2019·Journal of Bacteriology·Corinne R HutfilzCharmain T Courcelle

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