Enzymatic production of defined chitosan oligomers with a specific pattern of acetylation using a combination of chitin oligosaccharide deacetylases

Scientific Reports
Stefanie Nicole HamerStephan Kolkenbrock

Abstract

Chitin and chitosan oligomers have diverse biological activities with potentially valuable applications in fields like medicine, cosmetics, or agriculture. These properties may depend not only on the degrees of polymerization and acetylation, but also on a specific pattern of acetylation (PA) that cannot be controlled when the oligomers are produced by chemical hydrolysis. To determine the influence of the PA on the biological activities, defined chitosan oligomers in sufficient amounts are needed. Chitosan oligomers with specific PA can be produced by enzymatic deacetylation of chitin oligomers, but the diversity is limited by the low number of chitin deacetylases available. We have produced specific chitosan oligomers which are deacetylated at the first two units starting from the non-reducing end by the combined use of two different chitin deacetylases, namely NodB from Rhizobium sp. GRH2 that deacetylates the first unit and COD from Vibrio cholerae that deacetylates the second unit starting from the non-reducing end. Both chitin deacetylases accept the product of each other resulting in production of chitosan oligomers with a novel and defined PA. When extended to further chitin deacetylases, this approach has the potential...Continue Reading

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Citations

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Datasets Mentioned

BETA
AAF94439.1
BAD85943.1
CAB11942.1

Methods Mentioned

BETA
acetylation
electrophoresis
light scattering
size-exclusion chromatography
PCR
Protein Assay
flow microfiltration
size exclusion chromatography

Software Mentioned

AutoDock VINA
Data Analysis
AutoDockTools
BLASTn
GenScript
BLASTx
VINA
CloneManager

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