PMID: 16636462Apr 26, 2006Paper

Enzymatic production of highly soluble myricitrin glycosides using beta-galactosidase

Bioscience, Biotechnology, and Biochemistry
Ryosuke ShimizuMasamitsu Moriwaki

Abstract

Myricitrin, a botanical flavonol glycoside, could be a useful ingredient of functional foods, cosmetics, and medicines because of its high anti-oxidative activity. However, due to its insolubility in water, it has a limited range of use. To improve this solubility, we glycosylated myricitrin by an enzymatic transglycosylate reaction. Myricitrin was galactosylated by beta-galactosidase from Bacillus circulans using lactose as a sugar donor. The reaction product was 480 times more soluble than myricitrin. Four myricitrin galactosides were isolated from the reaction products by column chromatography, and their molecular structures were identified by using ESI-MS, 1H-NMR, 13C-NMR, 1H-1H COSY, 1H-13C HMQC and 1H-13C HMBC analysis. The solubility of these four myricitrin galactosides was more than 3.9 x 10(3) fold that of myricitrin, and each had similar anti-oxidative activity to that of myricitrin.

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Citations

Feb 11, 2011·Bioscience, Biotechnology, and Biochemistry·Jingyuan SongKazuhiro Nakanishi
Mar 6, 2007·Biochemical and Biophysical Research Communications·Lili LuYumei Li
Sep 14, 2014·Bioprocess and Biosystems Engineering·Kyung-Hwan Jung, Hyang-Yeol Lee
Apr 1, 2017·Applied Microbiology and Biotechnology·Tingting QiLili Lu
Jul 25, 2018·International Journal of Molecular Sciences·Kristýna SlámováKateřina Valentová
Mar 9, 2013·Archives of Pharmacal Research·Allia M AbdouAshraf B Abdel-Naim
Aug 7, 2010·Journal of Agricultural and Food Chemistry·Go-Eun KimDoman Kim

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