PMID: 9532781Apr 9, 1998Paper

Enzymatic properties of a Ginkgo biloba endo-beta-N-acetylglucosaminidase and N-glycan structures of storage glycoproteins in the seeds

Bioscience, Biotechnology, and Biochemistry
Y KimuraS Takagi

Abstract

An endo-beta-N-acetylglucosaminidase has been purified to homogeneity from mature seeds of Ginkgo biloba. The molecular mass of the endo-beta-N-acetylglucosaminidase, named Endo-GB, was estimated to be around 63 kDa by SDS-PAGE and around 62 kDa by Hiprep S-200 chromatography, respectively. The substrate specificity has been explored with regard to the pyridylaminated N-glycans. Several high mannose-type sugar chains bearing alpha-1,2-mannosyl residue(s), Man9-6GlcNAc2-PA, were the most favored substrates followed by Man5GlcNAc2-PA and a typical hybrid-type structure (GlcNAc1Man5Glc NAc2-PA) which does not bear an alpha-1,2-mannosyl residue. On the contrary, endo-GB could hardly hydrolyze the common core pentasaccharide of N-glycan (Man3GlcNAc2-PA) and the xylose-containing sugar chains (Man4-3Xyl1Glc NAc2-PA, Man3Fuc1Xyl1GlcNAc2-PA) being widely distributed in plant glycoproteins. Furthermore, we analyzed the structures of N-glycans conjugated to storage glycoproteins in the mature Ginkgo seeds to see the occurrence of endogenous substrates for Endo-GB. The structural analysis showed, however, only xylose-containing type N-glycans (Man3Fuc1Xyl1GlcNAc2 (95%) and Man3Xyl1 GlcNAc2 (5%)), which can not be substrate for Endo-GB, pr...Continue Reading

References

May 23, 1976·Biochemical and Biophysical Research Communications·S ChienY Li
Aug 1, 1990·Agricultural and Biological Chemistry·A KondoT Ikenaka
Feb 16, 1987·European Journal of Biochemistry·T H PlummerA L Tarentino
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Mar 1, 1995·Bioscience, Biotechnology, and Biochemistry·Y KimuraM Yonekura
Feb 1, 1996·Bioscience, Biotechnology, and Biochemistry·Y KimuraS Takagi
Dec 24, 1997·Bioscience, Biotechnology, and Biochemistry·Y KimuraS Takagi
Jan 1, 1997·Bioscience, Biotechnology, and Biochemistry·Yoshinobu KimuraTomonori Shiraishi

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Citations

Nov 11, 2008·Bioscience, Biotechnology, and Biochemistry·Kosuke NakamuraYoshinobu Kimura
Jan 9, 2009·Bioscience, Biotechnology, and Biochemistry·Kosuke NakamuraYoshinobu Kimura
Aug 10, 2006·Glycoconjugate Journal·Tadashi Suzuki, Yoko Funakoshi
Mar 17, 2015·Cellular and Molecular Life Sciences : CMLS·Yoichiro HaradaTadashi Suzuki
Oct 26, 2001·Bioscience, Biotechnology, and Biochemistry·Y KimuraM Kimura
Oct 31, 2000·Bioscience, Biotechnology, and Biochemistry·Y Kimura, E Kitahara
Dec 25, 2004·Bioscience, Biotechnology, and Biochemistry·Kwan Kit WooYoshinobu Kimura
Apr 19, 2002·Biochimica Et Biophysica Acta·Yoshinobu KimuraMikio Nishimura
Aug 28, 2021·Frontiers in Plant Science·Megumi MaedaYoshinobu Kimura

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