Enzymatic properties of neuraminidases from Arthrobacter ureafaciens

Journal of Biochemistry
Y UchidaT Sugimori

Abstract

Neuraminidase I and neuraminidase II from Arthrobacter ureafaciens were characterized. As determined by gel filtration on Ultrogel AcA 44, the molecular weights of neuraminidases I and II were 51,000 and 39,000, respectively. Neuraminidases I and II were similar to each other in their enzymatic properties except for the substrate specificities towards gangliosides and erythrocyte stroma. Their optimal pHs were between 5.0 and 5.5 with N-acetylneuraminosyl-lactose or bovine submaxillary mucin as substrates, but with colominic acid as a substrate, the pH optimum was between 4.3 and 4.5. They were most active around 53 degrees C, were stable between pH 6.0 and 9.0, and were thermostable up to 50 degrees C. They did not require Ca2+ for activity and were not inhibited by EDTA. They were inhibited only slightly or not at all by p-chloromercuribenzoic acid of Hg2+. Both neuraminidases I and II were able to hydrolyze the alpha-ketosidic linkage of N-glycolylneuraminic acid as well as that of N-acetylneuraminic acid, and were able to liberate substantially all of the sialic acid from various kinds of substrates. However, they cleaved only about 50% of the sialic acid from bovine submaxillary mucin. The saponification of bovine submaxil...Continue Reading

References

Jul 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yasuhiro KurodaShozo Izui
Feb 12, 2008·Current Protocols in Molecular Biology·L D Powell, A P Varki
May 6, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gregory R MoeBecca A Flitter
Jul 17, 2009·Biology of Reproduction·Kenji YamatoyaKiyotaka Toshimori
Oct 5, 2012·Journal of Virology·Johnna M FriersonTerence S Dermody
Dec 22, 2017·Critical Reviews in Biotechnology·Justin Bryan Goh, Say Kong Ng
Apr 1, 1991·Glycoconjugate Journal·D HeuermannR Schauer
Jan 1, 1990·Applied Biochemistry and Biotechnology·K B LeeR J Linhardt
Oct 6, 2007·Biological & Pharmaceutical Bulletin·Hyoung Jin Kim, Hong-Jin Kim
Jun 20, 2012·Preparative Biochemistry & Biotechnology·Yumiko ShimuraKenichi Hatanaka
Oct 22, 2008·Bioscience Reports·Tara A Cartwright, Ruth A Schwalbe
Aug 8, 2012·Glycoconjugate Journal·Lorena Sánchez-FelipeIsabel Muñoz-Barroso
Dec 24, 2010·Anatomia, histologia, embryologia·I HabataA Tsukise
Oct 1, 1991·Oral Microbiology and Immunology·T TakeshitaS Shizukuishi
May 1, 1990·Journal of Cellular Biochemistry·D R ThomsenA P Elhammer
Nov 7, 2007·The Journal of Veterinary Medical Science·Noriyuki TakabatakeIkuo Igarashi
Jul 16, 2011·The Journal of Biological Chemistry·Xuezheng SongDavid F Smith
Mar 26, 2015·Applied Biochemistry and Biotechnology·Rumyana Todorova EnevaIgnat Abrashev
Mar 13, 2015·Biology of Reproduction·Viswanadhapalli SuryavathiJohn C Herr

Related Concepts

Cations, Divalent
NeuAc-2-8-NeuAc
Mucin-1 protein
Stroma
colominic acid
Bos taurus
Calcium ion
Hot Temperature
Sialic Acid Assay
Gangliosides

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