Enzymatic removal of nitric oxide catalyzed by cytochrome c' in Rhodobacter capsulatus

Journal of Bacteriology
R CrossJames W B Moir

Abstract

Cytochrome c' from Rhodobacter capsulatus has been shown to confer resistance to nitric oxide (NO). In this study, we demonstrated that the amount of cytochrome c' synthesized for buffering of NO is insufficient to account for the resistance to NO but that the cytochrome-dependent resistance mechanism involves the catalytic breakdown of NO, under aerobic and anaerobic conditions. Even under aerobic conditions, the NO removal is independent of molecular oxygen, suggesting cytochrome c' is a NO reductase. Indeed, we have measured the product of NO breakdown to be nitrous oxide (N(2)O), thus showing that cytochrome c' is behaving as a NO reductase. The increased resistance to NO conferred by cytochrome c' is distinct from the NO reductase pathway that is involved in denitrification. Cytochrome c' is not required for denitrification, but it has a role in the removal of externally supplied NO. Cytochrome c' synthesis occurs aerobically and anaerobically but is partly repressed under denitrifying growth conditions when other NO removal systems are operative. The inhibition of respiratory oxidase activity of R. capsulatus by NO suggests that one role for cytochrome c' is to maintain oxidase activity when both NO and O(2) are present.

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Citations

May 31, 2007·Current Microbiology·Khaled M Khleifat, Ahmed H Al-Mustafa
May 11, 2002·Journal of Bacteriology·Muna F AnjumJames W B Moir
Jun 7, 2005·Journal of Bacteriology·Peter S ChoiJames P Shapleigh
Oct 19, 2010·Biophysical Chemistry·Paolo AscenziFabio Polticelli
Feb 13, 2007·FEBS Letters·Bradley O ElmoreAlan B Hooper
Jul 31, 2012·Biochimica Et Biophysica Acta·Jörg Simon, Martin G Klotz
Dec 26, 2003·Critical Reviews in Biochemistry and Molecular Biology·Daniel J Arp, Lisa Y Stein
Oct 25, 2016·Environmental Science and Pollution Research International·Awais ShakoorYouhua Ma
Jun 24, 2018·Applied and Environmental Microbiology·Qiu MengHaichun Gao
Dec 26, 2001·The Journal of Biological Chemistry·Anne M GardnerPaul R Gardner
Dec 13, 2006·The Journal of Biological Chemistry·Sergei G KruglikMichel Negrerie
Jan 26, 2005·Biochemical Society Transactions·W M HustonJ W B Moir
Apr 9, 2019·Metallomics : Integrated Biometal Science·Michel Négrerie
Jan 26, 2005·Biochemical Society Transactions·R K Poole
Apr 29, 2008·Biochemistry·Wojciech BialekAndrzej Szczepaniak
Jun 15, 2021·Bioscience, Biotechnology, and Biochemistry·Sotaro FujiiYoshihiro Sambongi
Feb 5, 2013·Journal of the American Chemical Society·Byung-Kuk YooMichel Negrerie
May 26, 2005·Journal of the American Chemical Society·Marcelo A MartíDario A Estrin
Jun 15, 2006·Chemical Reviews·Raymond WeissJames Terner

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