PMID: 5257960May 1, 1969Paper

Enzymatic repair of DNA, 1. Purification of two enzymes involved in the excision of thymine dimers from ultraviolet-irradiated DNA

Proceedings of the National Academy of Sciences of the United States of America
J C KaplanL Grossman

Abstract

Two nucleases that catalyze the excision of photoproducts from UV-irradiated DNA have been extensively purified from M. luteus (M. lysodeikticus). The first enzyme, an endonuclease, has been purified 5000-fold and is free of conflicting nuclease activities. It introduces single-strand breaks into irradiated DNA but does not act on native or single-stranded DNA. The purified enzyme is activated but not dependent on Mg(++) and has an approximate molecular weight of 15,000. Photoproduct excision is absolutely dependent on the second enzyme, a magnesium requiring exonuclease. This enzyme, which has been purified 1000-fold, is devoid of conflicting nucleases. It hydrolyzes irradiated and unirradiated denatured DNA at the same rate, but has no activity on RNA. It only acts on double-stranded DNA which has been both irradiated and pretreated with the endonuclease. The combined action of the endo- and exonuclease results in the quantitative removal of photoproduct regions from UV-irradiated DNA. Approximately five nucleotides are released for every single-strand incision.

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Citations

Jan 11, 1979·Molecular & General Genetics : MGG·J Auerbach, P Howard-Flanders
Oct 1, 1977·Zeitschrift Für Krebsforschung Und Klinische Onkologie. Cancer Research and Clinical Oncology·H W Thielmann
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Dec 25, 2007·Cell Research·Errol C Friedberg
Dec 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·S Yasuda, M Sekiguchi
Oct 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·M C Paterson, R B Setlow
May 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·P K Cooper, P C Hanawalt
May 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·A Braun, L Grossman
Dec 17, 2008·The Journal of Biological Chemistry·Raquel Sussman
Jun 1, 1975·Nucleic Acids Research·A CaputoA Clanciulli
Dec 1, 1977·Nucleic Acids Research·R Hecht, H W Thielmann
Jun 25, 1979·Nucleic Acids Research·G Ludwig, H W Thielmann
Oct 1, 1976·Photochemistry and Photobiology·A Guialis, R A Deering
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Jan 21, 1997·Proceedings of the National Academy of Sciences of the United States of America·S Shiota, H Nakayama
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Jun 15, 1978·European Journal of Biochemistry·J SvachulováJ Velemínský
Feb 7, 1973·Nature: New Biology·R B Setlow, W L Carrier

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