PMID: 11920669Mar 29, 2002Paper

Enzymatic responses of human deciduous pulpal fibroblasts to dental restorative materials

Journal of Biomedical Materials Research
Chern-Chin ChenShun-Te Huang

Abstract

The purpose of this study was to evaluate the responses of succinic dehydrogenase (SDH) and alkaline phosphatase (ALP) activities of human deciduous teeth pulpal fibroblasts (HDPF) to dental restorative materials. Tested materials included Z100 (3M), Dyract (Dentsply), FujiII (GC), and FujiIILC (GC). IRM (Dentsply) and culture medium (MD) alone were used as positive and negative controls, respectively. Specimens 6 mm (diameter) x 3 mm were prepared in accordance with manufacturers' instructions. For light-cured materials, specimens were light cured for 40 s on both sides under a celluloid strip. For chemical-cured materials, specimens were allowed to set at room temperature for 15 min. The specimens were immersed in 1 mL of culture medium without serum for 24 h at room temperature. The extracts were filtered through 0.22-mm filters. HDPF (10,000 cells/well) was incubated with 100 microL of extract and 20 % FBS in a 96-well plate for 24 h in a 37 degrees, 5 % CO(2) incubator. Six wells per material were prepared. Optical density (OD) of SDH and ALP of HDPF were measured by a spectrophotometer. The means were analyzed by ANOVA and then a Duncan Test. The ranking of OD of SDH was IRM < FujiIILC < FujiII = Z100 < Dyract < MD (p < 0...Continue Reading

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Citations

Jan 7, 2005·Clinical Oral Investigations·A S Al-HiyasatM M Milhem
Nov 28, 2007·Clinical Oral Investigations·Michel Goldberg
Jan 8, 2013·Toxicology International·Saurabh K GuptaAditya B Pant
Mar 8, 2014·Journal of Indian Prosthodontic Society·Muaiyed Mahmoud Buzayan, Norsiah Binti Yunus
Dec 17, 2014·Journal of Prosthodontic Research·Yoshio YamagaShuichi Nomura
Dec 14, 2005·Journal of Biomedical Materials Research. Part a·Satoshi ImazatoShigeyuki Ebisu

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