Enzymatic Self-Biodegradation of Poly(l-lactic acid) Films by Embedded Heat-Treated and Immobilized Proteinase K.

Biomacromolecules
QiuYuan HuangTadahisa Iwata

Abstract

Non-biodegradable microplastics have become a global problem. We propose a new enzyme-embedded biodegradable plastic that can be self-biodegraded anytime and anywhere. Proteinase K from Tritirachium album was embedded in poly(l-lactic acid) (PLLA). The PLLA solution-cast film with embedded proteinase K showed weight loss of 78% after 96 h incubation. In addition, PLLA extruded films embedding immobilized proteinase K encapsulated in polyacrylamide were produced at 200 °C and embedded-enzyme degradation was monitored. Immobilized proteinase K embedded in the extruded film maintained its degradation activity and degraded the PLLA film from inside to make small holes and cavities, suggesting that immobilization is a powerful technique to prepare thermoforms with embedded enzymes. The rate of embedded-enzyme degradation was accelerated by dividing the film into smaller pieces, which can be regarded as a model experiment for biodegradation of microplastics. Various biodegradable plastics with specific embedded enzymes will contribute to solve global environmental problems.

References

Jan 11, 2005·Biomacromolecules·Robert W Lenz, Robert H Marchessault
May 29, 2015·Biotechnology, Biotechnological Equipment·Nur Royhaila MohamadRoswanira Abdul Wahab

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Citations

Apr 20, 2021·Biomacromolecules·Angelique F GreeneKate Parker
Jun 15, 2021·International Journal of Biological Macromolecules·Bruna Coelho de AndradeClaucia Fernanda Volken de Souza

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