Enzymatic Study of Linoleic and Alpha-Linolenic Acids Biohydrogenation by Chloramphenicol-Treated Mixed Rumen Bacterial Species

Frontiers in Microbiology
Annabelle MeynadierFrancis Enjalbert

Abstract

In the rumen, dietary polyunsaturated fatty acids (PUFA) are reduced by a multistage reaction called biohydrogenation (BH). BH leads to a high proportion of saturated fat in ruminant products, but also products some potential bioactive intermediates like conjugated linoleic and linolenic acids. BH is composed of two kinds of reactions: first an isomerization of PUFA followed by reductions (two for linoleic acid, C18:2n-6; three for α-linolenic acid, C18:3n-3). There is little knowledge about BH enzymes as BH bacterial species are the subject of a lot of studies. Nevertheless, both aspects must be explored to control BH and enhance the fatty acids profile of ruminant products. In the present study, an alternative approach was developed to study the enzymes produced in vivo by mixed ruminal bacteria, using inactivation of bacteria by chloramphenicol, an inhibitor of protein synthesis in prokaryotes, before in vitro incubation. To study C18:2n-6 and C18:3n-3 BH several experiments were used: (1) with different incubation durations (0 to 3) to estimate average rates and efficiencies of all BH reactions, and intermediates production; and (2) with different initial quantities of PUFA (0.25 to 2 mg) to estimate Michaelis-Menten enzyma...Continue Reading

References

Aug 1, 1973·Journal of the Science of Food and Agriculture·C G HarfootJ H Moore
Oct 31, 2006·Antonie van Leeuwenhoek·Margarida R G MaiaR John Wallace
Mar 23, 2012·Animal : an International Journal of Animal Bioscience·A ZenedA Troegeler-Meynadier
Jan 13, 2015·Applied Microbiology and Biotechnology·Florence PrivéSharon A Huws
Apr 19, 2016·The British Journal of Nutrition·Anne M HonkanenKevin J Shingfield
May 31, 2017·Journal of Applied Microbiology·F EnjalbertA Meynadier

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Citations

Aug 31, 2018·Microbiology and Molecular Biology Reviews : MMBR·Ana S SalsinhaLuis M Rodríguez-Alcalá

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Software Mentioned

SPSS
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