Enzymatic synthesis of 2'-deoxyguanosine with nucleoside deoxyribosyltransferase-II

Bioscience, Biotechnology, and Biochemistry
Kiyoshi OkuyamaToshitada Noguchi

Abstract

Nucleoside deoxyribosyltransferase-II (NdRT-II) of Lactobacillus helveticus, which catalyzes the transfer of a glycosyl residue from a donor deoxyribonucleoside to an acceptor base, has a broad specificity for the acceptor bases. Six-substituted purines were found to be substrates as acceptor bases for NdRT-II. Using this property of the enzyme, we established a practical procedure for enzymatic synthesis of 2'-deoxyguanosine (dGuo), consisting of the transglycosylation from thymidine to 6-substituted purine (2-amino-6-chloropurine; ACP) instead of natural guanine and the conversion of 2-amino-6-chloropurine-2'-deoxyriboside (ACPdR) to dGuo with bacterial adenosine deaminase. Through the successive reactions, dGuo was synthesized in high yield.

Citations

Mar 27, 2013·Applied Microbiology and Biotechnology·A Fresco-TaboadaJ Fernández-Lucas
Apr 9, 2011·Applied Microbiology and Biotechnology·Jesús Fernández-LucasMiguel Arroyo
Jun 21, 2006·Biotechnology Letters·Nobuyuki HorinouchiSakayu Shimizu
Dec 27, 2011·Bioresource Technology·Jesús Fernández-LucasMiguel Arroyo
Sep 21, 2007·Biochimica Et Biophysica Acta·Yukiko MiyamotoShigeru Chohnan
Jul 28, 2012·Biotechnology Progress·Eliana C De BenedettiJorge A Trelles
Nov 3, 2010·Journal of Zhejiang University. Science. B·Qing-bao DingChun-yan Zhang
Dec 5, 2008·FEMS Microbiology Letters·Rosario MédiciAdolfo M Iribarren
Jun 16, 2009·Bioorganic & Medicinal Chemistry Letters·Rosario MédiciElizabeth S Lewkowicz
Apr 28, 2006·Chembiochem : a European Journal of Chemical Biology·Laura L Grochowski, T Mark Zabriskie
Dec 22, 2007·Biotechnology and Bioengineering·J Fernández-LucasJ V Sinisterra

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.