Enzymatic synthesis of chitosan-gelatin antimicrobial copolymer and its characterisation

Journal of the Science of Food and Agriculture
Lu-Yan SangGui-Luo Zhang

Abstract

Schiff bases can be formed by reaction between acylamino and the amino group with microbial transglutaminase (MTGase) as catalyst and used in protein crosslinking in the food industry. A novel chitosan-gelatin copolymer for coating meat products can be synthesised using MTGase as catalyst, with the characteristics of both chitosan and gelatin. The aim of the present study was to synthesise and characterise chitosan-gelatin antimicrobial copolymer. The yield of copolymer increased with increasing gelatin/chitosan ratio, while bacteriostasis of Staphylococcus aureus by the copolymer decreased, showing that its bacteriostatic ability depended on the amino group in chitosan. Under optimal synthesis conditions of 50 degrees C, pH 6 and 40 min reaction time the copolymer yield was 64.5% at a gelatin/chitosan ratio of 0.6. Bacteriostasis of S. aureus by 10 g kg(-1) copolymer solution was 70% of that by chitosan. A novel biological dressing frame material with excellent biocompatibility and antibacterial properties has been successfully prepared. The results of this study provide a background for further investigation of potential applications of chitosan-gelatin copolymer in the areas of agriculture and food.

References

Jan 1, 1985·Methods in Enzymology·J E Folk, S I Chung
Dec 16, 1998·Toxicological Sciences : an Official Journal of the Society of Toxicology·T NarahashiW D Atchison

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Citations

Apr 9, 2014·Carbohydrate Polymers·Lihong FanShuhua Liu
Jul 6, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Julius A Edson, Young Jik Kwon
Jun 1, 2016·Enzyme and Microbial Technology·Nadine KarakiJordane Jasniewski
Feb 27, 2016·Journal of the Science of Food and Agriculture·Miao Fu, Xin-Huai Zhao
Jun 9, 2015·Colloids and Surfaces. B, Biointerfaces·Ildyko KovachJoachim Koetz
Feb 20, 2014·Macromolecular Bioscience·Marcelo A da SilvaCécile A Dreiss
Dec 11, 2014·Journal of the Science of Food and Agriculture·Zhen-Ling Luo, Xin-Huai Zhao
Jul 21, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Luminita MarinMihail Barboiu
Aug 26, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yinglei ZhangShouwen Zhang
Feb 26, 2019·Applied Microbiology and Biotechnology·Soo Khim Chan, Theam Soon Lim
Jun 8, 2018·Advanced Science·Shuqiang LiXuesi Chen

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