Abstract
For synthesizing glycerol carbonate (GC) by a reaction between glycerol (GL) and dimethyl carbonate (DMC), a lipase immobilized on magnetic organosilica nanoflowers was prepared and utilized as a biocatalyst. Candida antarctica lipase B (CALB) was chosen as a model enzyme for preparing an immobilized biocatalyst (CALB@nanoflowers). The obtained CALB@nanoflowers was characterized using scanning electron microscopy, transmission electron microscopy, and confocal laser scanning microscopy. Effects of GL/DMC molar ratio, biocatalyst amount, temperature, surfactant and molecular sieve addition, and reaction time on the conversion of GL and the selectivity of CALB@nanoflowers were investigated. The optimal catalytic performance (yield of GC: 88.66% and conversion of GL: 94.24%) was achieved under the condition of 1:20 molar ratio of GL to DMC with 0.2 g of molecular sieves added at 50 °C for 24 h. After recycling seven times, the CALB@nanoflowers maintained over 79% of its initial activity and the yield of GC was 70.31%.
References
Feb 26, 1982·Biochemical and Biophysical Research Communications·V Kasche, B Galunsky
Jan 1, 1994·Advances in Protein Chemistry·Z S Derewenda
Sep 23, 2003·Journal of Molecular Biology·José M MancheñoJuan A Hermoso
Jan 29, 2008·Chemical Society Reviews·Chun-Hui Clayton ZhouG Q Max Lu
Apr 15, 1991·Biotechnology and Bioengineering·D B VolkinA M Klibanov
May 27, 2010·Bioprocess and Biosystems Engineering·Kyung Hwa LeeEun Yeol Lee
Mar 15, 2012·Biotechnology for Biofuels·Fangxia YangRuncang Sun
Jul 5, 2012·Enzyme and Microbial Technology·Hongsub JungChulhwan Park
Oct 13, 2012·Chemical Society Reviews·Rafael C RodriguesRoberto Fernández-Lafuente
Jan 29, 2013·Biomaterials·Youhui LinXiaogang Qu
Mar 14, 2013·Chemical Society Reviews·Francesco Secundo
Mar 23, 2013·Chemical Society Reviews·Maurice C R FranssenJohan P M Sanders
Aug 21, 2013·Biotechnology Advances·Panagiota-Yiolanda StergiouEmmanuel M Papamichael
Sep 24, 2013·Chemical Society Reviews·Yanlong Gu, François Jérôme
Mar 4, 2014·Bioresource Technology·Yoon Ju JoEun Yeol Lee
Mar 15, 2015·Enzyme and Microbial Technology·Evelin A ManoelRoberto Fernandez-Lafuente
Sep 9, 2015·Biochemistry·Themistoklis ZisisKerstin G Blank
Oct 28, 2015·Nanoscale·Chen HouMingzhu Liu
Dec 20, 2015·Advanced Materials·Zhaowei ChenXiaogang Qu
Mar 5, 2016·Advanced Materials·Yu Chen, Jianlin Shi
Mar 24, 2016·Bioresource Technology·Xiaolan LuoYebo Li
Nov 23, 2016·Enzyme and Microbial Technology·Jose J Virgen-OrtízRoberto Fernandez-Lafuente
Dec 23, 2016·ACS Applied Materials & Interfaces·Sanjay K S PatelJung-Kul Lee
Jan 6, 2017·Journal of Agricultural and Food Chemistry·Jiandong CuiShiru Jia
Jun 13, 2017·Bioresource Technology·Jechan LeeEilhann E Kwon
Jul 18, 2017·International Journal of Biological Macromolecules·Kh PashangehG Absalan
Oct 7, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Zhengyang ZhaoLizhong He
Citations
Jun 11, 2019·Frontiers in Chemistry·Pascale de CaroSophie Thiebaud-Roux
Sep 7, 2019·International Journal of Biological Macromolecules·Nathalia Saraiva RiosLuciana Rocha Barros Gonçalves
Sep 16, 2019·Protein Expression and Purification·Lisette Van TasselLloyd W Ruddock
Apr 6, 2021·Journal of Oleo Science·Kajal JaiswalVirendra K Rathod
May 10, 2021·Talanta·Jia LiuYan-Ping Shi