Enzymatic synthesis of organic-polymer-grafted DNA

Chemistry : a European Journal
Anna Baccaro, Andreas Marx

Abstract

To create bioorganic hybrid materials, interdisciplinary work in the fields of chemistry, biology and materials science is conducted. DNA block copolymers are promising hybrid materials due to the combination of properties intrinsic to both the polymer and the nucleic acid blocks. Until now, the coupling of DNA and organic polymers has been exercised post-synthetically in solution or on solid support. Herein, we report the first enzyme-catalysed synthesis of DNA-organic polymer chimeras. For this purpose, four novel 2'-deoxyuridine triphosphates carrying polymer-like moieties linked to the nucleobase were synthesised. Linear polyethylene glycol monomethyl ethers of different sizes (1) and branched polyamido dendrons with varying terminal groups (2) were chosen as building blocks. We investigated the ability of DNA polymerases to accept the copolymers in comparison to the natural substrate and showed, through primer extensions, polymerase chain reactions and rolling circle amplification, that these building blocks could serve as a surrogate for the natural thymidine. By this method, DNA hybrid materials with high molecular weight, modification density, and defined structure are accessible.

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Citations

Dec 3, 2010·Proceedings of the National Academy of Sciences of the United States of America·Samra ObeidAndreas Marx
Mar 8, 2016·Accounts of Chemical Research·Audrey Hottin, Andreas Marx
Mar 28, 2013·Chembiochem : a European Journal of Chemical Biology·Pavel KielkowskiMichal Hocek
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Nov 20, 2018·Chembiochem : a European Journal of Chemical Biology·Ivo Sarac, Marcel Hollenstein
Aug 25, 2010·Chembiochem : a European Journal of Chemical Biology·Nadine Staiger, Andreas Marx
Aug 14, 2013·Angewandte Chemie·Jitka DadováMichal Hocek
Feb 4, 2011·Chembiochem : a European Journal of Chemical Biology·Hana Macíčková-CahováMichal Hocek
Jul 22, 2014·Chembiochem : a European Journal of Chemical Biology·Marcel Hollenstein, Christian J Leumann
Sep 19, 2015·Chembiochem : a European Journal of Chemical Biology·Michaela MačkováMichal Hocek
May 31, 2018·Nucleic Acids Research·Jevgenija JakubovskaRolandas Meškys
Jul 7, 2012·Chemical Communications : Chem Comm·Samra ObeidAndreas Marx
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Sep 21, 2011·Organic & Biomolecular Chemistry·Yan WangAndreas Marx
Apr 10, 2012·Bioorganic & Medicinal Chemistry Letters·Bastian HolzbergerAndreas Marx

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