PMID: 16622282Apr 20, 2006Paper

Enzymatic synthesis of prebiotic oligosaccharides

Applied Biochemistry and Biotechnology
Maria C RabeloSueli Rodrigues

Abstract

Prebiotic oligosaccharides are nondigestible carbohydrates that can be obtained by enzymatic synthesis. Glucosyltransferases can be used to produce these carbohydrates through an acceptor reaction synthesis. When maltose is the acceptor a trisaccharide composed of one maltose unit and one glucose unit linked by an alpha-1,6-glycosidic bond (panose) is obtained as the primer product of the dextransucrase acceptor reaction. In this work, panose enzymatic synthesis was evaluated by a central composite experimental design in which maltose and sucrose concentration were varied in a wide range of maltose/sucrose ratios in a batch reactor system. A partially purified enzyme was used in order to reduce the process costs, because enzyme purification is one of the most expensive steps in enzymatic synthesis. Even using high maltose/sucrose ratios, dextran and higher-oligosaccharide formation were not avoided. The results showed that intermediate concentrations of sucrose and high maltose concentration resulted in high panose productivity with low dextran and higher-oligosaccharide productivity.

Citations

Feb 27, 2007·Bioprocess and Biosystems Engineering·Clarice M A ChagasSueli Rodrigues
Jun 2, 2012·Indian Journal of Microbiology·Maria Cristiane RabeloSueli Rodrigues
Sep 6, 2014·Journal of Food Science and Technology·Isabel Moreira da SilvaSueli Rodrigues
Jul 18, 2009·Bioresource Technology·Maria Cristiane RabeloSueli Rodrigues
Sep 8, 2015·Journal of Food Science and Technology·Raquel Macedo Dantas CoelhoSueli Rodrigues
Aug 23, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Natália G GraebinRafael C Rodrigues
Sep 29, 2018·Food Science and Biotechnology·Hoon KimKwang-Soon Shin
Jul 17, 2018·Chemical Reviews·Liuqing WenPeng George Wang

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