May 1, 1976

Enzymatic synthesis of two fucose-containing glycolipids with fucosyltransferases of human serum

European Journal of Biochemistry
T Pacuszka, J Kościelak

Abstract

Lacto-N-neotetraosylceramide incubated with human serum fucosyltransferase preparations gave rise to two fucoglycolipids. The faster migrating fucoglycolipid I on the basis of its thin-layer chromatographic mobility, susceptibility to alpha(1 leads to 2) fucosidase from Trichomonas foetus, radio-immunoprecipitation with Ulex europeus lectin and studies with Oh (Bombay) sera was identified as H-active glycolipid (H-I). The most probable structure of fucoglycolipid II should be that with fucose linked alpha(1 leads to 3) to N-acetylglucosamine. Lactosylceramide, ceramide trihexoside and globoside were not substrates for human serum fucosyltransferases. Lacto-N-neotetraosyl ceramide served as a fucose acceptor for all serum preparations tested while asialoganglioside was a substrate only when serum preparations containing H-gene dependent alpha-2-L-fucosyltransferase were used. With asialoganglioside only one radioactive reaction product was formed.

Mentioned in this Paper

Glycosphingolipids
Ceramides
Ceramide trihexoside
Glycolipids
Cytolipins
Globosides
Phosphoglycerate Dehydrogenase Activity
Fucosyltransferase
CDw17 antigen
Lectin Activity

About this Paper

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