Enzymatically cross-linked gelatin-phenol hydrogels with a broader stiffness range for osteogenic differentiation of human mesenchymal stem cells

Acta Biomaterialia
Lishan WangMotoichi Kurisawa

Abstract

An injectable hydrogel system, composed of gelatin-hydroxyphenylpropionic acid (Gtn-HPA) conjugates chemically cross-linked by an enzyme-mediated oxidation reaction, has been designed as a biodegradable scaffold for tissue engineering. In light of the role of substrate stiffness on cell differentiation, we herein report a newly improved Gtn hydrogel system with a broader range of stiffness control that uses Gtn-HPA-tyramine (Gtn-HPA-Tyr) conjugates to stimulate the osteogenic differentiation of human mesenchymal stem cells (hMSCs). The Gtn-HPA-Tyr conjugate was successfully synthesized through a further conjugation of Tyr to Gtn-HPA conjugate by means of a general carbodiimide/active ester-mediated coupling reaction. Proton nuclear magnetic resonance and UV-visible measurements showed a higher total phenol content in the Gtn-HPA-Tyr conjugate than that content in the Gtn-HPA conjugate. The Gtn-HPA-Tyr hydrogels were formed by the oxidative coupling of phenol moieties catalyzed by hydrogen peroxide (H(2)O(2)) and horseradish peroxidase (HRP). Rheological studies revealed that a broader range of storage modulus (G') of Gtn-HPA-Tyr hydrogel (600-26,800 Pa) was achieved using different concentrations of H(2)O(2), while the G' of th...Continue Reading

References

May 23, 2001·Journal of Agricultural and Food Chemistry·G OudgenoegC Laane
Jan 5, 2002·Advanced Drug Delivery Reviews·Allan S Hoffman
Apr 12, 2002·Journal of Controlled Release : Official Journal of the Controlled Release Society·A Hatefi, B Amsden
Jul 27, 2002·Analytical Chemistry·Anja SchmidtUrsula Bilitewski
Nov 12, 2002·Biomacromolecules·Xiao Zheng ShuGlenn D Prestwich
Apr 11, 2003·Proceedings of the National Academy of Sciences of the United States of America·M P LutolfJ A Hubbell
Dec 4, 2003·Biomaterials·Xiao Zheng ShuGlenn D Prestwich
Dec 2, 2004·Cell Motility and the Cytoskeleton·Tony YeungPaul A Janmey
Jan 25, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Aurore SchneiderCatherine Picart
Aug 23, 2006·Cell·Adam J EnglerDennis E Discher
May 18, 2007·Advanced Drug Delivery Reviews·James D KretlowAntonios G Mikos
Jun 5, 2007·Advanced Drug Delivery Reviews·Joerg K Tessmar, Achim M Göpferich
Aug 30, 2008·American Journal of Physiology. Cell Physiology·Andrew S RowlandsJustin J Cooper-White
Jan 6, 2009·Journal of Controlled Release : Official Journal of the Controlled Release Society·Fan LeeMotoichi Kurisawa
Oct 10, 2009·Annals of Biomedical Engineering·Stephanie Nemir, Jennifer L West
Nov 3, 2009·Biomaterials·Min HuJackie Y Ying

❮ Previous
Next ❯

Citations

Feb 13, 2016·Trends in Biotechnology·Barbara J KlotzFerry P W Melchels
Jun 13, 2014·Cell Biology International·Jieling ShanXiaobing Zou
Mar 10, 2015·Acta Biomaterialia·Chia-Hui ChuangYing-Chieh Chen
Nov 26, 2014·Journal of Biomaterials Science. Polymer Edition·Zhiping PengLei Chen
May 31, 2014·Stem Cells Translational Medicine·Jessica E FrithJustin J Cooper-White
May 2, 2018·Biomaterials Science·Mehdi KhanmohammadiJafar Ai
Feb 3, 2016·Biomedical Materials·Myron Spector, Teck Chuan Lim
Oct 8, 2015·Macromolecular Bioscience·Yong Yao ChunCleo Choong
Oct 27, 2018·International Journal of Molecular Sciences·Jui-Yang LaiDavid Hui-Kang Ma
Jul 21, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Shalini V GohilLakshmi S Nair
Oct 17, 2019·Journal of Biomedical Materials Research. Part a·Nima FirouziDavoud Kazemi
May 14, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Pin-Yu WangGwo-Jang Wu
May 14, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yunki LeeKi Dong Park
Jan 28, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Phuong Le ThiKi Dong Park
Nov 17, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Phuong Le ThiKi Dong Park
Feb 27, 2021·Biomaterials Science·Feifei WangHongbo Zeng
Aug 30, 2017·International Journal of Biological Macromolecules·Shalini V GohilLakshmi S Nair
Jun 22, 2021·ACS Biomaterials Science & Engineering·Syuan-Yu MaoYing-Chieh Chen
Dec 4, 2014·Biomacromolecules·Clementine PradalJustin J Cooper-White
Dec 8, 2017·ACS Omega·Vladimíra MoulisováGloria Gallego Ferrer
Dec 12, 2016·ACS Biomaterials Science & Engineering·Benjamin P PartlowDavid L Kaplan
May 6, 2017·ACS Applied Materials & Interfaces·Yunki LeeKi Dong Park
Aug 31, 2021·Acta Biomaterialia·Soichiro FujiwaraYuichi Ohya

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.