Enzymatically synthesized polyaniline layer for extension of linear detection region of amperometric glucose biosensor

Biosensors & Bioelectronics
Asta Kausaite-MinkstimieneArunas Ramanavicius

Abstract

In this article a new method for fabrication of enzymatic electrodes suitable for design of amperometric glucose biosensor and/or anode of biofuel cell powered by glucose is presented. Glucose oxidase (GOx) E.C. 1.1.3.4. from Penicillium vitale was immobilized on the carbon rod electrode by cross-linking it with glutaraldehyde (GOx-electrode). Catalytic activity of immobilized GOx was exploited for polymerisation of aniline by taking a high concentration of hydrogen peroxide produced during the catalytic action of immobilized GOx and locally lowered pH due to the formation of gluconic acid; it created optimal conditions for the polymerisation of aniline. The GOx layer was self-encapsulated within formed polyaniline (PANI) matrix (GOx/PANI-electrode). Properties of the GOx/PANI-electrode have been studied and results were compared with GOx-electrode. The results show that the upper detection limit of glucose using GOx-electrode was dramatically changed by the formation of PANI layer. An increase in the upper detection limit, optimal pH region for operation and stability of GOx based electrode modified by PANI was detected when comparing that of an unmodified GOx-electrode.

Citations

Jul 13, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Karolis LeonaviciusArunas Ramanavicius
May 19, 2012·Lab on a Chip·Lauren Matlock-Colangelo, Antje J Baeumner
Jan 23, 2016·Chemical Reviews·Shin-ichiro ShodaShiro Kobayashi
Jan 25, 2011·Biosensors & Bioelectronics·Huanfen YaoBabak A Parviz
Dec 15, 2010·Biosensors & Bioelectronics·Yasemin OztekinArunas Ramanavicius
Aug 28, 2015·ACS Applied Materials & Interfaces·Mubarak AliWolfgang Ensinger
Jun 24, 2017·Nanoscale Research Letters·Libo ZhuQuanjun Liu
Aug 5, 2017·Chemické Zvesti·Gordana Ćirić-MarjanovićPeter Walde
Feb 14, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Lei ZhangDan Xiao

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