Enzyme activities and gene expression of starch metabolism provide insights into grape berry development

Horticulture Research
Xudong ZhuJinggui Fang

Abstract

Grapes are categorized as a non-climacteric type of fruit which its ripening is not associated to important rises in respiration and ethylene synthesis. The starch metabolism shares a certain role in the carbohydrate metabolic pathways during grape berry development, and is regarded as an important transient pool in the pathway of sugar accumulation. However, the comprehensive role of starch and its contribution to the quality and flavor of grape berry have not been explored thoroughly. In this study, the expression levels of genes enzyme activities and carbohydrate concentrations related to starch metabolism, were analyzed to understand the molecular mechanism of starch accumulation during grape berry development. The results indicated that starch granules in grape berry were located at the chloroplast in the sub-epidermal tissues, acting as the temporary reserves of photosynthetic products to meet the needs for berry development, and relatively high starch contents could be detected at véraison stage. Moreover, both ADP-glucose pyrophosphorylase (EC 2.7.7.27) and sucrose phosphate synthase (EC 2.3.1.14) involved in starch synthesis displayed elevated gene expression and enzymes activities in the sub-epidermal tissue, while α-...Continue Reading

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Citations

Aug 4, 2018·Electrophoresis·Nor Azreen Mohd JamilJameel R Al-Obaidi
Jan 24, 2019·Plant, Cell & Environment·Noam ReshefNurit Agam

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Methods Mentioned

BETA
transmission electron microscopy
electron microscopy
PCR

Software Mentioned

MEGA
Oligo
InterProScan
Primer Premier
BioXM
ExPASy
Clustal Omega
Gene

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