Enzyme-assisted extraction of lipids directly from the culture of the oleaginous yeast Rhodosporidium toruloides

Bioresource Technology
Guojie JinZongbao K Zhao

Abstract

Lipids produced by oleaginous microorganisms are a potential feedstock for biodiesel production and chemical synthesis. Yet, the costs of microbial lipids remain high, partially because the lipid recovery process is tedious and costly. In the present study, enzyme-assisted extraction of lipids from the culture of the yeast Rhodosporidium toruloides was carried out. With a heat pre-treatment with microwave, enzymatic treatment with the recombinant β-1,3-glucomannanase, plMAN5C, and extraction with ethyl acetate, 96.6% of the total lipids were extracted from R. toruloides cells at room temperature and atmospheric pressure directly from the culture without dewatering. Therefore, this process could significantly reduce energy consumption and costs for lipids extraction from the yeast.

References

Apr 24, 2008·Biotechnology and Bioengineering·Bruce E Rittmann
Apr 24, 2009·Bioresource Technology·Jae-Yon LeeHee-Mock Oh
Jun 6, 2009·Bioresource Technology·Cuimin HuZongbao K Zhao
Mar 6, 2010·Journal of Industrial Microbiology & Biotechnology·Xin ZhaoZongbao K Zhao
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Mar 8, 2011·Journal of Biotechnology·Jintao LinZongbao K Zhao
Nov 25, 2011·Enzyme and Microbial Technology·Fan YangZongbao K Zhao

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Citations

May 2, 2013·Biotechnology Advances·Jungmin KimJi-Won Yang
Jul 19, 2014·Journal of Biotechnology·Isabel Espinosa-GonzalezDavid C Bressler
Apr 9, 2015·International Journal of Molecular Sciences·Dongqin WangMin Zhong
Dec 3, 2014·Bioresource Technology·Xiaobing YangZongbao Kent Zhao
Apr 15, 2016·Applied Biochemistry and Biotechnology·Zhi-Jian TanChao-Yun Wang
Jul 5, 2015·Bioresource Technology·Kazuhiko KurosawaAnthony J Sinskey
Mar 19, 2014·Bioresource Technology·Xue BaiSteven Pratt
Dec 9, 2014·Trends in Biotechnology·Mingjie JinVenkatesh Balan
Oct 21, 2016·Frontiers in Microbiology·Katrin OchsenreitherChristoph Syldatk
Feb 9, 2017·International Journal of Biological Macromolecules·Fengping JiaoLe Jia
Jan 13, 2018·Annual Review of Food Science and Technology·Lauryn G ChanJuliana Maria Leite Nobrega de Moura Bell
Feb 22, 2018·Biotechnology Progress·Ahasanul KarimDomenico Pirozzi
May 26, 2016·Nature Communications·Yongjin J ZhouJens Nielsen
Nov 15, 2018·Applied Microbiology and Biotechnology·Diandra Albuquerque Lopes Costa, Edivaldo Ximenes Ferreira Filho
Sep 1, 2017·Journal of Separation Science·Ting Hu, Jin-Lan Zhang
May 8, 2020·Applied Biochemistry and Biotechnology·Qiang LiQitian Huang
Oct 6, 2020·Frontiers in Bioengineering and Biotechnology·Olga GorteAude Silve
Jul 1, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alok PatelLeonidas Matsakas
Apr 16, 2019·FEMS Yeast Research·Xiangjian LiuZongbao Kent Zhao
Jan 9, 2021·Bioresource Technology·E Tomás-PejóC González-Fernández
Jan 23, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·André DelavaultKatrin Ochsenreither
Dec 29, 2017·ACS Synthetic Biology·Yongjin J ZhouJens Nielsen

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