Enzyme-assisted extraction of polyphenol from edible lotus (Nelumbo nucifera) rhizome knot: Ultra-filtration performance and HPLC-MS2 profile

Food Research International
Zhenzhou ZhuFrancisco J Barba

Abstract

Rhizome knot is always wasted as useless and inedible part of lotus root, despite its abundance of polyphenols. In this work, enzyme-assisted extraction followed by ultra-filtration was investigated to recover polyphenols from rhizome knot. Cellulase and pectinase treatment enhanced the polyphenols extraction. The 100 kDa membrane resulted in better filtration yield than 50 kDa membrane, 3.84% and 3.37%, respectively. With 100 kDa membrane, the highest filtration yield (4.08%) was achieved with a rotational speed of 600 rpm, TMP of 0.3 MPa and pH of 5. Satisfied permeate turbidity (<3 NTU) and polyphenol transmission (>90%) were obtained under these conditions. The main polyphenols identified in both rhizome knot extract and permeate were: chlorogenic acid, B-type procyanidin dimer·H2O, (+)-Catechin, B-type procyanidin dimer, (-)-Epicatechin, propyl gallate·H2O, caffeic acid, (-)-Epicatechin-3-gallate, and rutin. Membrane fouling led to the most important resistance (58% of total resistance). Scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) evidenced that protein accumulation was the main fouling cause.

Citations

Feb 6, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhenzhou ZhuEugene Vorobiev
Apr 17, 2019·Critical Reviews in Food Science and Nutrition·Le WenBrijesh K Tiwari
Apr 14, 2020·Journal of Food Biochemistry·Shuyi LiOlusola Lamikanra
Aug 24, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shuyi LiFrancisco J Barba
Nov 7, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhili MaDeyuan Li
Jul 30, 2019·Journal of Food Biochemistry·Juanmei ZhangWenjing Ma
Nov 25, 2018·Bioprocess and Biosystems Engineering·Rui ZhangEugène Vorobiev
Nov 5, 2020·Bioprocess and Biosystems Engineering·Bin YuanAiling Liu
May 1, 2021·Pharmaceuticals·Tapan BehlDomenico Montesano

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