Enzyme-catalyzed mechanism of isoniazid activation in class I and class III peroxidases.

The Journal of Biological Chemistry
Roberta PierattelliKatherine A Brown

Abstract

There is an urgent need to understand the mechanism of activation of the frontline anti-tuberculosis drug isoniazid by the Mycobacterium tuberculosis catalase-peroxidase. To address this, a combination of NMR spectroscopic, biochemical, and computational methods have been used to obtain a model of the frontline anti-tuberculosis drug isoniazid bound to the active site of the class III peroxidase, horseradish peroxidase C. This information has been used in combination with the new crystal structure of the M. tuberculosis catalase-peroxidase to predict the mode of INH binding across the class I heme peroxidase family. An enzyme-catalyzed mechanism for INH activation is proposed that brings together structural, functional, and spectroscopic data from a variety of sources. Collectively, the information not only provides a molecular basis for understanding INH activation by the M. tuberculosis catalase-peroxidase but also establishes a new conceptual framework for testing hypotheses regarding the enzyme-catalyzed turnover of this compound in a number of heme peroxidases.

References

Aug 1, 1991·Protein Engineering·K DarwishT L Poulos
Jul 1, 1991·Journal of Biomolecular NMR·C EcclesK Wüthrich
Apr 30, 1990·European Journal of Biochemistry·N C Veitch, R J Williams
Mar 1, 1985·Antimicrobial Agents and Chemotherapy·H A ShoebA J Merola
Sep 1, 1970·Journal of General Microbiology·F G Winder, P B Collins
May 1, 1995·Biochemical Society Transactions·N C Veitch
Apr 4, 1995·Biochemistry·W R Patterson, T L Poulos
Apr 4, 1997·The Journal of Biological Chemistry·R S Magliozzo, J A Marcinkeviciene
Apr 1, 1997·Journal of Molecular Graphics & Modelling·R M Esnouf
Dec 24, 1997·Nature Structural Biology·M GajhedeT L Poulos
Jul 17, 1998·Current Opinion in Chemical Biology·A T Smith, N C Veitch
Jul 18, 2001·Journal of the American Chemical Society·J BodiguelB Jamart-Grégoire
Sep 21, 2001·Biochemical and Biophysical Research Communications·C JakopitschC Obinger
Dec 31, 2002·The Journal of Biological Chemistry·Salem ChouchaneRichard S Magliozzo
Mar 12, 2003·Journal of Molecular Biology·Xavi CarpenaIgnacio Fita
Mar 18, 2003·Nature Structural Biology·Katherine H SharpEmma Lloyd Raven
May 7, 2003·Biochemistry·Christa JakopitschChristian Obinger
Jul 3, 2004·The Journal of Biological Chemistry·Thomas BertrandKatherine A Brown
Jul 9, 2004·Biochemistry·Katherine H SharpEmma Lloyd Raven

❮ Previous
Next ❯

Citations

Nov 28, 2013·Archives of Biochemistry and Biophysics·Olive J NjumaDouglas C Goodwin
Nov 30, 2005·Dalton Transactions : an International Journal of Inorganic Chemistry·Nigel A J EadyKatherine A Brown
Jun 18, 2010·The Journal of Biological Chemistry·Ben WisemanPeter C Loewen
Mar 22, 2013·Protein Engineering, Design & Selection : PEDS·Tianlei YingZhong-Xian Huang
Mar 9, 2010·Archives of Biochemistry and Biophysics·Andrea GumieroEmma Lloyd Raven
Nov 1, 2006·Molecular Microbiology·Graham S Timmins, Vojo Deretic
May 20, 2014·European Journal of Medicinal Chemistry·Filomena MartinsMiguel Viveiros
Sep 11, 2016·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Ameeruddin Nusrath UnissaNagamiah Selvakumar
Dec 7, 2007·The Journal of Biological Chemistry·Clive MetcalfePeter C E Moody
Jul 3, 2004·The Journal of Biological Chemistry·Thomas BertrandKatherine A Brown
Sep 22, 2005·Journal of the American Chemical Society·Reza A GhiladiPaul R Ortiz de Montellano

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antitubercular Agents (ASM)

Antitubercular agents are pharmacologic agents for treatment of tuberculosis. Discover the latest research on antitubercular agents here.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Antitubercular Agents

Antitubercular agents are pharmacologic agents for treatment of tuberculosis. Discover the latest research on antitubercular agents here.