PMID: 518208Nov 1, 1979Paper

Enzyme comparative study of spermatozoa and seminal plasma in normal and subfertile man

Archives of Andrology
J F GuerinJ C Czyba

Abstract

A semiquantitative colorimetric micromethod (APIZYM) was used to study the enzyme profiles of seminal plasma and of spermatozoa. Reactions with 65 different substrates are simultaneously tested in a single specimen. These substrates (principally naphtolic) allow the detection of hydrolytic enzymes (esterases, phosphatases, and peptidases) and of dehydrogenases potentially involved in sperm metabolism and in the process of fertilization. The usual sperm enzymes were regularly observed: C3-C4 esterases, amino acid arylamidases, acrosine, phosphatases, glutamyl transpeptidase, and various osidases. Among the dehydrogenases we observed a striking predominance of the enzymes of the hexose monophosphate shunt and of LDH. Seminal plasma has an enzyme pattern very similar to that of spermatozoa except for the absence of acrosine and of some dehydrogenases. This unexpected similarity is discussed. The gametes from subfertile donors do not at first sight differ in their enzyme pattern from those from fertile donors. Moreover, we found no marked differences between zymograms of seminal plasma from normal, subfertile, or even azoospermic patients. Deep freezing does not modify the hydrolytic enzymes of human sperm either quantitatively of ...Continue Reading

References

Jan 1, 1978·Archives of Andrology·P ChapdelaineJ Mailhot
Oct 1, 1971·Biology of Reproduction·R N Peterson, M Freund
Nov 1, 1973·Biology of Reproduction·G Oliphant, B G Brackett
Mar 1, 1969·American Journal of Obstetrics and Gynecology·S J Behrman, D R Ackerman
Jan 1, 1969·Histochemie. Histochemistry. Histochimie·F P Altmann
Feb 1, 1964·British Journal of Pharmacology and Chemotherapy·E W HORTON, C J THOMPSON

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Citations

Nov 6, 2015·BioMed Research International·Rui-Xiang FengNian-Qing Lü
Aug 1, 1983·International Journal of Andrology·D FabbroU Eppenberger
May 1, 1992·Archives of Andrology·L C García DíezJ M Miralles
Jul 1, 1991·Archives of Andrology·M KamadaS S Koide
Jan 1, 1982·Andrologia·J M Gonzalez Buitrago, L C Garcia Diez
Mar 1, 1988·Journal of Andrology·T G CooperE Nieschlag

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