PMID: 11312692Apr 21, 2001Paper

Enzyme conjugation to the polysaccharide chitosan: smart biocatalysts and biocatalytic hydrogels

Bioconjugate Chemistry
R Vazquez-DuhaltG F Payne

Abstract

Laccase from Coriolopsis gallica was conjugated to the renewable biopolymer chitosan using carbodiimide chemistry. The laccase-chitosan conjugate was observed to offer three unique properties. First, the laccase-chitosan conjugate displayed pH-responsive behavior such that the conjugate was soluble and active under acidic conditions, but precipitated when the pH was raised toward neutrality. Second, the laccase-chitosan conjugate was more stable than free laccase at extreme pHs. At pH 1, the inactivation rate constant (k(in)) for the soluble laccase-chitosan conjugate was 20-fold less than that for free laccase. At pH 13, k(in) for the insoluble laccase-chitosan conjugate was nearly 3-fold less than that for free laccase. Finally, the laccase-chitosan conjugate could be cross-linked under mild conditions to create biocatalytic hydrogels. Potential benefits for enzyme-chitosan conjugates are discussed.

References

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Citations

Jun 16, 2006·Bioprocess and Biosystems Engineering·Leissy GómezReynaldo Villalonga
Nov 7, 2003·Journal of Controlled Release : Official Journal of the Controlled Release Society·Canh Le TienMircea-Alexandru Mateescu
Jan 20, 2004·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·J BergerR Gurny
Apr 6, 2013·Chemical Reviews·Wipa SugintaAlbert Schulte
Feb 6, 2014·Chemical Reviews·María L VillalongaReynaldo Villalonga
Oct 14, 2014·Chemical Communications : Chem Comm·Sarra El IchiDonald K Martin
Dec 15, 2010·Biosensors & Bioelectronics·Javier Martinez-OrtizRafael Vazquez-Duhalt
Mar 17, 2005·International Journal of Biological Macromolecules·Gary DelanoyJian Yu
Aug 4, 2011·Enzyme Research·Alexandre ArsenaultJ Peter Jones
Jun 20, 2016·Environmental Science and Pollution Research International·Rina D Koyani, Rafael Vazquez-Duhalt
Jul 31, 2003·Biotechnology and Bioengineering·Hyunmin YiWilliam E Bentley
Apr 24, 2007·Soft Matter·Gregory F Payne, Srinivasa R Raghavan
Mar 28, 2018·Bioprocess and Biosystems Engineering·Monika Osińska-JaroszukAnna Jarosz-Wilkołazka
Dec 1, 2017·Polymer International·Ying HaoXinqiao Jia
Sep 22, 2020·Journal of Forensic Sciences·Nemanja VučkovićNikola Milašinović
Mar 6, 2021·International Journal of Biological Macromolecules·F Rafiee, M Rezaee
Apr 6, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Li-Qun WuGregory F Payne
Aug 30, 2008·Analytical Chemistry·John NjagiSilvana Andreescu
Dec 28, 2006·Langmuir : the ACS Journal of Surfaces and Colloids·Rodolfo Cruz-SilvaJorge Romero-Garcia
Nov 15, 2005·Biomacromolecules·Hyunmin YiGregory F Payne

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