Enzyme engineering for enantioselectivity: from trial-and-error to rational design?

Trends in Biotechnology
Linda G OttenIsabel W C E Arends

Abstract

The availability of tailored enzymes is crucial for the implementation of biocatalysis in organic chemistry. Enantioselectivity is one key parameter defining the usefulness of an enzyme and, therefore, the competitiveness of the corresponding industrial process. Hence, identification of enzymes with high enantioselectivity in the desired transformation is important. Currently, this is achieved by screening collections and libraries comprising natural or man-made diversity for the desired trait. Recently, a variety of improved methods have been developed to generate and screen this diversity more efficiently. Here, we present and discuss the most important advances in both library generation and screening. We also evaluate future trends, such as moving from random evolution to more rational.

References

Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·W P Stemmer
Jun 4, 2002·Nature Structural Biology·Christopher A VoigtFrances H Arnold
Dec 20, 2002·Angewandte Chemie·Frédéric TaranCharles Mioskowski
Jan 4, 2003·Chembiochem : a European Journal of Chemical Biology·Dongxing ZhaManfred T Reetz
Jan 30, 2003·Analytical Chemistry·Erik Henke, Uwe T Bornscheuer
Aug 14, 2003·Chemistry : a European Journal·Patrick TielmannManfred T Reetz
Aug 21, 2003·Journal of Molecular Biology·Marcus D HughesAnna V Hine
Dec 18, 2003·Angewandte Chemie·Masayuki MatsushitaKim D Janda
Apr 14, 2004·Proceedings of the National Academy of Sciences of the United States of America·Manfred T Reetz
Jul 13, 2004·Trends in Biotechnology·Jean-Philippe Goddard, Jean-Louis Reymond
Aug 7, 2004·Current Opinion in Biotechnology·Stefan Lutz, Wayne M Patrick
Sep 11, 2004·Current Opinion in Biotechnology·Karl-Erich Jaeger, Thorsten Eggert
Sep 11, 2004·Current Opinion in Biotechnology·Jean-Philippe Goddard, Jean-Louis Reymond
Nov 13, 2004·Nucleic Acids Research·Jorge YáñezPaul Gaytán
Mar 16, 2005·Analytical Biochemistry·Thomas VanherckePeter Denolf
May 4, 2005·Trends in Biotechnology·Krista L Morley, Romas J Kazlauskas
Jun 16, 2005·Journal of the American Chemical Society·Sangeeta DeyDavid B Berkowitz
Jul 27, 2005·Current Opinion in Structural Biology·Hans LeemhuisFlorian Hollfelder
Feb 16, 2006·Journal of the American Chemical Society·Anders HambergKarl Hult
May 27, 2006·Combinatorial Chemistry & High Throughput Screening·Tuck Seng WongUlrich Schwaneberg
Jun 22, 2006·Chemistry : a European Journal·Manfred T ReetzAndreas Vogel
Aug 2, 2006·Molecular BioSystems·Hana MatsushitaKim D Janda
Aug 23, 2006·Critical Reviews in Biotechnology·Jasjeet Kaur, Rohit Sharma
Oct 13, 2006·Chemical Communications : Chem Comm·Jennifer AndexerThorsten Eggert
Oct 13, 2006·Applied Microbiology and Biotechnology·Ren-Chao ZhengYin-Chu Shen
Nov 30, 2006·Chembiochem : a European Journal of Chemical Biology·Manfred T ReetzNikolaj Otte
Feb 27, 2007·Nature Biotechnology·Richard J FoxGjalt W Huisman
Mar 3, 2007·Chemistry : a European Journal·Anders HambergKarl Hult
Apr 5, 2007·Nature Protocols·Dominique Böttcher, Uwe T Bornscheuer
May 24, 2007·Journal of the Royal Society, Interface·Ashok A DenizEdward A Lemke
Jan 29, 2008·Trends in Biotechnology·Richard J Fox, Gjalt W Huisman
Mar 8, 2008·Science·Lin JiangDavid Baker
Apr 3, 2008·Chembiochem : a European Journal of Chemical Biology·Ykelien L BoersmaWim J Quax
May 31, 2008·Angewandte Chemie·Stefan BeckerHarald Kolmar
Jun 25, 2008·Annual Review of Biophysics·Christian JäckelDonald Hilvert
Aug 23, 2008·Applied Microbiology and Biotechnology·Anett Kirschner, Uwe T Bornscheuer
Nov 11, 2008·Chemical Communications : Chem Comm·Manfred T ReetzLayla Fernández
Nov 14, 2008·Proceedings of the National Academy of Sciences of the United States of America·Eugene AntipovAlexander M Klibanov

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Citations

Aug 17, 2010·Applied and Environmental Microbiology·Moran BroukAyelet Fishman
May 21, 2013·Enzyme and Microbial Technology·Bradford SullivanJon D Stewart
Dec 17, 2014·Microbial Cell Factories·Viviane Paula MartiniNadia Krieger
Nov 26, 2015·Journal of Industrial Microbiology & Biotechnology·Youran LiGuiyang Shi
Sep 28, 2012·Science China. Life Sciences·ChuanPeng LiuQiMing Zhou
Jul 6, 2010·Trends in Biotechnology·Richard A GrossWenhua Lu
May 21, 2010·Trends in Biotechnology·Misha V Golynskiy, Burckhard Seelig
Jun 20, 2015·Applied Microbiology and Biotechnology·Ya-Shan ChengRey-Ting Guo
Apr 17, 2013·Biotechnology Journal·Zhen Chen, An-Ping Zeng
Nov 10, 2015·Chemical Reviews·Yongxi ZhaoChunhai Fan
Aug 28, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Yan FangZhi-Kang Xu
Jun 23, 2011·Chemical Reviews·Mélanie Hall, Andreas S Bommarius
Feb 6, 2014·Chemical Reviews·María L VillalongaReynaldo Villalonga
Feb 5, 2013·ACS Chemical Biology·Christopher M PirieParayil Kumaran Ajikumar
Dec 20, 2011·Proceedings of the National Academy of Sciences of the United States of America·Anders G SandströmJan-E Bäckvall
Jun 7, 2014·Chemical Communications : Chem Comm·Dirk HoltmannFrank Hollmann
Apr 24, 2012·Chembiochem : a European Journal of Chemical Biology·Yosephine GumulyaManfred T Reetz
Jun 29, 2011·Chembiochem : a European Journal of Chemical Biology·Peter James Baker, Jin Kim Montclare
Apr 8, 2011·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Shreenath PrasadManfred T Reetz
Jul 19, 2012·Chemistry : a European Journal·Zhi-Gang ZhangManfred T Reetz
Aug 3, 2017·Critical Reviews in Biotechnology·Carla SilvaArtur Cavaco-Paulo
Sep 16, 2018·Applied and Environmental Microbiology·Shalev GihazAyelet Fishman
Feb 1, 2019·Bioscience Reports·Clare F Megarity
May 14, 2011·Chembiochem : a European Journal of Chemical Biology·Manfred T Reetz, Huabao Zheng
Jun 20, 2012·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Manfred T Reetz
Oct 19, 2013·Chembiochem : a European Journal of Chemical Biology·Loreto P ParraManfred T Reetz
Aug 23, 2019·Electrophoresis·Flora W Y Chiu, Stavros Stavrakis
Aug 30, 2014·Chemical Communications : Chem Comm·Gheorghe-Doru RoibanManfred T Reetz
Nov 24, 2011·Chembiochem : a European Journal of Chemical Biology·Jan G E van LeeuwenDick B Janssen
Mar 15, 2011·Current Protocols in Protein Science·Evangelia G Chronopoulou, Nikolaos E Labrou

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