Enzyme-functionalized silica nanoparticles as sensitive labels in biosensing

Analytical Chemistry
Yafeng WuSongqin Liu

Abstract

A novel strategy for sensitive detection of biomarkers using horseradish peroxidase (HRP)-functionalized silica nanoparticles as the label is presented. The enzyme-functionalized silica nanoparticles were fabricated by coimmobilization of HRP and alpha-fetoprotein antibody (anti-AFP, the secondary antibody, Ab2), a model protein, onto the surface of SiO(2) nanoparticles using gamma-glycidoxypropyltrimethoxysilane (GPMS) as the linkage. Through "sandwiched" immunoreaction, the enzyme-functionalized silica nanoparticle labels were brought close to the surface of gold substrates, as confirmed by the scanning electron microscopy (SEM) images. Enhanced detection sensitivity was achieved where the large surface area of SiO(2) nanoparticle carriers increased the amount of HRP bound per sandwiched immunoreaction. The electrochemical and chemiluminescence measurement showed 29.5- and 61-fold increases in detection signals, respectively, in comparison with the traditional sandwich immunoassay. The improved particle synthesis using a "seed-particle growth" route yielded particles of narrow size distribution, which allowed consistent loading of HRP and anti-AFP on each microsphere and ensured subsequent immunosensing possessed high sensiti...Continue Reading

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Citations

Jan 15, 2014·Biosensors & Bioelectronics·Jiachao YuSongqin Liu
Feb 20, 2014·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Hao YangSongqin Liu
Jan 20, 2012·Chemical Communications : Chem Comm·Se Won BaeJong-In Hong
Feb 23, 2011·International Journal of Molecular Sciences·Linghua MengLei Zheng
Dec 17, 2011·International Journal of Molecular Sciences·Ning GanLei Zheng
Oct 21, 2011·Journal of Automated Methods & Management in Chemistry·Ning GanLei Zheng
Jan 1, 2010·Expert Opinion on Medical Diagnostics·Alfredo de la Escosura-Muñiz, Arben Merkoçi
Apr 17, 2012·Current Opinion in Chemical Biology·Haesik Yang
Aug 18, 2010·Clinica Chimica Acta; International Journal of Clinical Chemistry·Liyuan ChenSongqin Liu
Sep 4, 2015·Analytical and Bioanalytical Chemistry·Ju-Hwan OhJae-Seung Lee
Jul 9, 2013·Analytical Biochemistry·Sara NouraniSeyed Mehdi Boutorabi
May 6, 2017·Sensors·Mingfei PanShuo Wang
Mar 11, 2018·Journal of Molecular Modeling·Marjan A Nejad, Herbert M Urbassek

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