Enzyme histochemistry of the rat nasal mucosa embedded in cold glycol methacrylate

The American Journal of Anatomy
H W RandallK T Morgan


The nasal passages are anatomically complex, and while there have been a number of descriptions of nasal structure in many species, there is very little information available on the distribution of enzymes in the nasal mucosa. In rodents, this delicate mucosa is the first site within the respiratory tract to be exposed during inhalation toxicology studies designed to assess human risks from such exposures. However, the nasal mucosa presents problems for histologic preparation because it is encased in brittle bones. Because of recent interest in the nose as a target site, and findings from biochemical studies which indicate that the nose is very active metabolically, studies were carried out to determine the value of cold glycol methacrylate (GMA) processing for localization of nasal enzymes. For these studies, liver and kidney were used as positive controls. Published histochemical procedures for acid and alkaline phosphatase, adenosine triphosphatase, glucose-6-phosphatase, gamma-glutamyl transpeptidase, and naphthyl butyrate esterase were applied, with modifications, to undecalcified nasal passages of Fisher-344 rats. Frozen sections exhibited excellent enzyme preservation but very poor morphology, while GMA gave good enzyme ...Continue Reading


Mar 15, 1986·Toxicology and Applied Pharmacology·M S BogdanffyK T Morgan
Apr 1, 1985·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·L A BuckleyC S Barrow
Sep 1, 1971·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M Hoshino, H Kobayashi
Dec 1, 1972·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A E AshfordM E McCully
Aug 1, 1969·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A M RutenburgA M Seligman
Aug 1, 1984·The American Review of Respiratory Disease·K T MorganE A Gross
Jan 1, 1984·The American Journal of Anatomy·N A Monteiro-Riviere, J A Popp
Jul 1, 1984·Toxicology and Applied Pharmacology·L A BuckleyC S Barrow
Aug 1, 1982·Journal of Microscopy·D M Harvey
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Dec 31, 1997·Mutation Research·J R Thornton-Manning, A R Dahl
Sep 1, 1991·Experimental Lung Research·M BonnefoiK T Morgan
Nov 1, 1990·Toxicology and Applied Pharmacology·D A KellerK T Morgan
Feb 1, 1991·Toxicology and Applied Pharmacology·B A Trela, M S Bogdanffy
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Apr 1, 1990·Environmental Health Perspectives·K T Morgan, T M Monticello
Jan 1, 1991·Critical Reviews in Toxicology·A R Dahl, W M Hadley
Jan 1, 1991·Toxicologic Pathology·H R BrownK T Morgan
Apr 1, 1990·Environmental Health Perspectives·M S Bogdanffy

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alpha naphthyl butyrate esterase
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