Enzyme immobilized in a packed-bed reactor: kinetic parameters and mass transfer effects.

Biotechnology and Bioengineering
W N MarrazzoB J McCoy

Abstract

To describe axial dispersion, particle film mass transfer, intraparticle diffusion, and the chemical reaction of the substrate for enzymes immobilized in porous particles in packed columns, we have developed mathematical models for first- and zero-order limits of Michaelis-Menten kinetics. Steady-state solutions were derived for both long and short column boundary conditions and for plug flow. Theory was compared to experiments by hydrolysis of sucrose catalyzed by invertase bound to porous glass particles. Steady-state conversions were measured for a range of flow rates. Pulse response experiments with inert packing were used to determine values of bed void fraction and particle porosity.

References

Dec 1, 1972·Biochemical Medicine·J C Powell, M Lever
Nov 1, 1972·Biotechnology and Bioengineering·D F Ollis
Sep 1, 1971·Biotechnology and Bioengineering·L BowskiW R Vieth
Sep 3, 1963·Biochimica Et Biophysica Acta·M V TRACEY

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Citations

Aug 1, 1986·Biotechnology and Bioengineering·R Lortie, D Thomas
Jul 1, 1983·Biotechnology and Bioengineering·D A Sirotti, A Emery
Nov 1, 1982·Biotechnology and Bioengineering·C O Malikkides, R H Weiland
Apr 1, 1982·Biotechnology and Bioengineering·V S Patwardhan, N G Karanth

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