PMID: 3745924Aug 21, 1986Paper

Enzyme immunoassay for human chorionic gonadotropin using monoclonal antibodies elicited with a synthetic peptide

Journal of Immunological Methods
B LonghiJ Caraux

Abstract

We recently described the generation of mouse monoclonal antibodies directed against hCG using a synthetic peptide immunogen: the carboxy-terminal peptide (CTP) of beta-hCG. The CTP, residues 109-145, is a portion unique to hCG and is not present in other glycoprotein hormones. These monoclonal antibodies react with beta-hCG-CTP and whole hCG but do not react with hLH. In the present study, a two-site sandwich ELISA specific for hCG has been developed using these CTP-induced monoclonal antibodies. Two monoclonal antibodies were paired: beta-hCG-CTP-a6 was immobilized to give a hCG-specific solid phase, and a biotinylated monoclonal antibody against beta-hCG was used as the indicator antibody. This second antibody was introduced during the hCG-capture step because of its agonist effect on hCG-capture by beta-hCG-CTP-a6 solid phase. This ELISA system can detect 2.2 IU/l hCG in a serum-free medium and 10.4 IU/l in a medium containing 20% human serum. We did not observe any cross-reactivity with hLH up to concentrations of 5000 IU/l. The hCG-ELISA correlated well (r = 0.99) with a control RIA in an assay of 78 human sera from healthy males, pregnant or non-pregnant females and from patients with choriocarcinoma, hydatidiform mole, ...Continue Reading

References

Aug 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·H C ChenG T Ross
Dec 1, 1982·The Journal of Clinical Endocrinology and Metabolism·H Suginami, A Kawaoi

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Citations

May 25, 1988·Journal of Immunological Methods·C SecchiS Ronchi
Oct 24, 2013·Analytical Chemistry·Xiaokang Ding, Kun-Lin Yang
Sep 13, 2005·Journal of Immunoassay & Immunochemistry·Anupam BasuSaumen Kumar Maitra

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