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Enzyme immunoassay for plasma 25-hydroxyvitamin D3 employing immunoaffinity chromatography as a pretreatment method

The Journal of Steroid Biochemistry and Molecular Biology

Jan 1, 1993

N KobayashiK Shimada

PMID: 8424897

Abstract

An enzyme immunoassay (EIA) of 25-hydroxyvitamin D3 [25(OH)D3] has been developed as a new methodology for measuring its plasma levels. Three anti-25(OH)D3 antibodies elicited against 25(OH)D3 3-hemisuccinate or -hemiglutarate conjugated with bovine serum albumin were used for this purp...read more

Mentioned in this Paper

Calcifediol, (3 beta,5E,7E)-Isomer
GLB1
ALB
Cross Reactions
Sephadex LH 20
Antigens
Immunoaffinity Chromatography
Enzyme Immunoassay
Immunosorbents
Beta-Galactosidase
Paper Details
References
  • References13
  • Citations3
12

Enzyme immunoassay for plasma 25-hydroxyvitamin D3 employing immunoaffinity chromatography as a pretreatment method

The Journal of Steroid Biochemistry and Molecular Biology

Jan 1, 1993

N KobayashiK Shimada

PMID: 8424897

DOI:

Abstract

An enzyme immunoassay (EIA) of 25-hydroxyvitamin D3 [25(OH)D3] has been developed as a new methodology for measuring its plasma levels. Three anti-25(OH)D3 antibodies elicited against 25(OH)D3 3-hemisuccinate or -hemiglutarate conjugated with bovine serum albumin were used for this purp...read more

Mentioned in this Paper

Calcifediol, (3 beta,5E,7E)-Isomer
GLB1
ALB
Cross Reactions
Sephadex LH 20
Antigens
Immunoaffinity Chromatography
Enzyme Immunoassay
Immunosorbents
Beta-Galactosidase

Related Papers

Paper Details
References
  • References13
  • Citations3
12
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