PMID: 2507458Jul 1, 1989Paper

Enzyme immunoassay of gastrin releasing peptide (GRP)-like immunoreactivity in milk

International Journal of Peptide and Protein Research
M TakeyamaH Yajima

Abstract

A sensitive and specific enzyme immunoassay (EIA) for gastrin releasing peptide (GRP)-like immunoreactivity was developed using enzyme-labeled antigen. The synthetic carboxy-terminal fragment of human GRP(12-27) was conjugated with beta-D-galactosidase for EIA. The minimum amount of GRP-like immunoreactivity detectable by this method was 0.24 femtomol/well (6 picomol/liter). The level of GRP-like immunoreactive substance in bovine foremilk was about 150 nanomol/liter, the level of which was more than hundredfold higher than that in normal milk or calf serum.

References

Jun 29, 1978·Nature·J WhartonA G Pearse
Sep 12, 1979·Biochemical and Biophysical Research Communications·T J McDonaldV Mutt
Nov 1, 1978·The Journal of Endocrinology·N S Whitworth, C E Grosvenor
Apr 15, 1985·European Journal of Biochemistry·H WernerY Koch
Jun 1, 1986·Chemical & Pharmaceutical Bulletin·S KunoH Yajima
Feb 15, 1987·Experientia·M PappC Scarpignato
Nov 27, 1985·Biochemical and Biophysical Research Communications·H WernerI Gozes
Sep 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·E R SpindelJ F Habener
Jun 2, 1980·Life Sciences·O Koldovsky
Sep 17, 1982·Biochimica Et Biophysica Acta·Z MoldoveanuK M Pruitt
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·E Rozengurt, J Sinnett-Smith
Feb 29, 1984·Biochemical and Biophysical Research Communications·N MinaminoH Matsuo
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G D Jahnke, L H Lazarus
Jul 29, 1983·Biochemical and Biophysical Research Communications·N MinaminoH Matsuo
Jul 1, 1982·Journal of Reproduction and Fertility·D B McClelland
Mar 24, 1980·Life Sciences·R R GalaA Turvey
Jan 1, 1977·Journal of Food Protection·T Richardson, Nina Mattarella

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Citations

Sep 6, 2005·Biological & Pharmaceutical Bulletin·Yuhki SatoMasaharu Takeyama
Mar 27, 2002·Biological & Pharmaceutical Bulletin·Takafumi NaitoMasaharu Takeyama
Jun 23, 2005·The Journal of Pharmacy and Pharmacology·Fumihiko KatagiriMasaharu Takeyama
Sep 2, 2006·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Hiroki Itoh
Jan 1, 1991·Trends in Endocrinology and Metabolism : TEM·E Hazum
Oct 15, 1991·Biochemical and Biophysical Research Communications·M TakeyamaN Fujii

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