PMID: 6166648Aug 1, 1981Paper

Enzyme-like immunosorbent assay (ELISA) for immunoglobulin G antibodies against insect venoms

The Journal of Allergy and Clinical Immunology
J A GrantJ Gillaspy

Abstract

IgG "blocking" antibodies were measured in patients receiving insect venom immunotherapy. The enzyme-linked immunosorbent assay (ELISA) described herein was found to be sensitive and reproducible. Results with ELISA correlated well with values obtained with a radioimmunoassay and with inhibition of the release of histamine from sensitive basophils. Also, specific antibody titers against phospholipase A and whole bee venom were correlated. Serial determinations of venom-specific IgG antibodies were made in 17 patients receiving Polistes wasp or bee venom immunotherapy. The majority of patients showed a rise in IgG antibodies, which peaked after administration of approximately 500 micrograms of venom. Only one out of 13 of these venom-treated patients had allergic symptoms after an insect sting while on maintenance therapy.

References

Jul 27, 1978·The New England Journal of Medicine·K J HuntL M Lichtenstein
Jul 1, 1979·The Journal of Allergy and Clinical Immunology·L M LichtensteinA K Sobotka
Jan 1, 1979·International Archives of Allergy and Applied Immunology·S MiyachiD M Kemeny
Feb 1, 1980·The Journal of Allergy and Clinical Immunology·P S Norman
Jan 1, 1980·Journal of Immunoassay·D de Savigny, A Voller

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Citations

May 10, 1985·Journal of Immunological Methods·R UrbanekD Samuel
Jun 1, 1988·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·M BorisS Weindorf
Sep 14, 1999·The Journal of Allergy and Clinical Immunology·T Midoro-HoriutiE G Brooks

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