Enzyme-linked immunoadsorbent assay (ELISA) for calcium-binding protein of human small intestine. A quantitative method for measuring calcium-binding protein in small intestinal biopsies

Clinica Chimica Acta; International Journal of Clinical Chemistry
M Staun

Abstract

A method for measuring calcium-binding protein (CaBP) in small intestinal biopsies of the human is reported. The assay is performed as a competitive enzyme-linked immunoadsorbent assay (ELISA). Pure CaBP and a cytosol fraction of human jejunum generate competitive displacement curves running in parallel thus demonstrating the antigen specificity of the assay. Non-specific displacement of anti-CaBP antibodies from the solid phase CaBP is negligible as demonstrated by the inability of cytosolic proteins of rat small intestine to produce a detectable response in the assay. The method has a detection limit of 3 ng CaBP (applied in 150 microliter) and a coefficient of variation of 9.8%. The ELISA method is applicable in the study of the role of CaBP in clinical disorders of the small bowel.

References

Oct 1, 1977·The Journal of Infectious Diseases·D E BidwellA Voller
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Jan 1, 1973·Vitamins and Hormones·R H Wasserman, R A Corradino
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May 6, 1966·Science·R H Wasserman, A N Taylor

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Citations

May 1, 1995·Calcified Tissue International·C HemmingsenK Olgaard
Apr 1, 1988·Calcified Tissue International·M StaunS Jarnum
Jul 30, 1987·Clinica Chimica Acta; International Journal of Clinical Chemistry·M Staun
Apr 1, 1991·Scandinavian Journal of Clinical and Laboratory Investigation·M StaunK Olgaard

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