PMID: 6989517May 1, 1980Paper

Enzyme-linked immunoassay for placental lactogen in human serum

Clinical Chemistry
D J MacDonaldD S Mack


We describe an enzyme-linked immunoassay for measuring human placental lactogen (HPL) in serum. After suitable dilution, sera are pipetted into the wells of polyvinyl microtitre plates previously coated with anti-HPL. After incubation the sera are decanted, and replaced by a conjugate of horseradish peroxidase and anti-HPL. An amount of enzyme proportional to the concentration of HPL in the serum is bound to the wells via an antibody--HPL--antibody bridge. Peroxidase activity is measured by oxidation of o-phenylenediamine, the resulting color being related to the concentration of HPL. Results correlate well with those by radioimmunoassay (r = 0.96). Our between-assay coefficient of variation was 13%; no discernible effect of protein was observed. This 4-h assay can be used to monitor placental function in pregnancy.

Related Concepts

Hydrogen-Ion Concentration
Peroxidase-Antiperoxidase Complex Technique
Human Placental Lactogen
Antenatal Screening Procedures

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