PMID: 39946Sep 1, 1979

Enzyme-linked immunosorbent assay for detection of Streptococcus pneumoniae antigen

Journal of Clinical Microbiology
S A HardingM A Sande

Abstract

An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of Streptococcus pneumoniae polysaccharide antigen in cerebrospinal fluid and serum. Sensitivity and specificity were determined for purified antigen preparations. Specificity was also evaluated in the rabbit meningitis model, and the sensitivity was compared to counterimmunoelectrophoresis, using the infected rabbits' cerebrospinal fluid and serum. The ELISA was a specific technique for detecting S. pneumoniae antigen. ELISA was 25 times more sensitive than counterimmunoelectrophoresis for purified antigen and resulted in an increased positivity of the cerebrospinal fluid and serum from infected rabbits. ELISA should prove very useful in the diagnosis of pneumococcal infections.

References

Jan 1, 1990·Scandinavian Journal of Infectious Diseases. Supplementum·M A Salih
Jan 1, 1986·Medical Microbiology and Immunology·C H EricssonI Sjögren
Oct 1, 1989·Epidemiology and Infection·M A SalihP Olcén
Jun 1, 1986·European Journal of Clinical Microbiology·A Balows
Feb 1, 1987·European Journal of Clinical Microbiology·S Lenthe-EboaF A Waldvogel

Related Concepts

Mycoplasma pneumoniae
Meningitis, Pneumococcal, Penicillin-Resistant
Streptococcus pneumoniae antigen
Cross Reactions
Cerebrospinal Fluid
Antigens, Bacterial
Antigens
Countercurrent Electrophoresis Measurement
Pneumococcal Infections
Enzyme-Linked Immunosorbent Assay

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