PMID: 8959667Nov 1, 1996Paper

Enzyme-linked immunosorbent assay for detection of feline serum amyloid A protein by use of immunological cross-reactivity of polyclonal anti-canine serum amyloid A protein antibody

The Journal of Veterinary Medical Science
T KajikawaS Sugii

Abstract

Immunological cross-reactivity between feline and canine serum amyloid A protein (SAA) was studied to establish enzyme-linked immunosorbent assay (ELISA) with heterologous antibody. Purified feline SAA formed a precipitin line in immunogel double diffusion with anti-canine SAA antibody. Immunological cross-reactivity was similarly observed in ELISA. In sandwich ELISA with anti-canine SAA antibody, a dose-response curve was obtained over the range of 2 micrograms/ml to 123 micrograms/ml of purified feline SAA. From the present findings, the sandwich ELISA is found to have high repeatability for quantitation of purified feline SAA and may be applicable to determine the serum concentration of feline SAA.

Citations

Aug 10, 2007·The Veterinary Journal·Saverio Paltrinieri
Mar 11, 2016·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Jenny StillerBarbara Kohn
Sep 16, 1999·The Journal of Veterinary Medical Science·K OhnoT Onishi
May 26, 2004·The Veterinary Journal·H MurataM Yoshioka

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