PMID: 8964612Jan 1, 1996Paper

Enzyme-linked immunosorbent assay for human MRP-8/MRP-14 proteins and their quantitation in plasma of hematological patients

Immunology Letters
G E IvanovV E Ivanov

Abstract

We developed a quantitative enzyme immunoassay for human MRP-8/MRP-14, neutrophil cytosolic proteins with calcium-binding and bacteriastatic properties. MRP-8/MRP-14 concentrations were measured in the plasma of 23 healthy volunteers and in 75 patients with hematological disorders. These proteins were detected in plasma of healthy blood donors with mean +/- standard deviation 167 +/- 114 ng/ml. MRP-8/MRP-14 plasma levels ranged from 435 to 13280 ng/ml in patients with chronic myeloid leukemia (CML), from 50 to 7570 ng/ml in chronic lymphoid leukemia (CLL), from 450 to 2790 ng/ml in polycythemia vera (PV), and were significantly higher than in healthy blood donors (P < 0.01 for CML and P < 0.05 for others). In CML patients MRP-8/MRP-14 levels strongly correlated with total blood WBC count (r = 0.82) and with neutrophilic granulocyte (NG) count (r = 0.80). Correlation of these values in PV amounted to r = 0.70 and r = 0.46, respectively. In CLL patients MRP-8/MRP-14 levels strongly correlated with total WBC count (r = 0.92), but not with the NG count. We suggest that MRP-8/MRP-14 quantitation may serve as a marker of neutrophil pool turnover in some hematological disorders.

References

Nov 1, 1991·Scandinavian Journal of Clinical and Laboratory Investigation·H Berner BerntzenP Mowinckel
Aug 9, 1991·Journal of Immunological Methods·A V PanyutichT Ganz
Jan 1, 1991·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·A G SembJ Vaage
Jan 1, 1968·Scandinavian Journal of Clinical and Laboratory Investigation·C E Mogensen
Feb 3, 1993·Journal of Immunological Methods·A V PanyutichT Ganz
May 1, 1965·The Journal of Clinical Investigation·J W ATHENSM M WINTROBE

❮ Previous
Next ❯

Citations

Sep 1, 2000·FEMS Immunology and Medical Microbiology·D V PechkovskyN I Misuno
Jun 1, 1997·Molecular Pathology : MP·B JohneI Dale
Jul 8, 2016·Adipocyte·Claude ForestPhilippe Noirez
May 1, 2013·The American Journal of Tropical Medicine and Hygiene·Israel MolinaAlbert Pahissa
Jan 24, 2014·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Mazher MohammedWilliam Blessing
Aug 5, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Youichirou OotsukaWilliam W Blessing
Mar 8, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Anna EskilssonAnders Blomqvist

❮ Previous
Next ❯

Related Concepts

Related Feeds

B-Cell Leukemia (Keystone)

B-cell leukemia includes various types of lymphoid leukemia that affect B cells. Here is the latest research on B-cell leukemia.