PMID: 2946597Sep 1, 1986Paper

Enzyme-linked immunosorbent assay for quantitative determination of the visual pigment rhodopsin in total-eye extracts

Experimental Eye Research
J J Schalken, W J de Grip

Abstract

A versatile, multispecies enzyme-linked immunosorbent assay for the rod visual pigment (rhod)opsin has been developed. For this quantitative inhibition assay a monospecific polyclonal antiserum is used which is elicited in rabbits against bovine rod outer-segment membranes. Detergent concentrations as high as 1.0% can be used in the assay with only a slight loss in sensitivity. The assay allows quantitative determination of the apoprotein opsin with a detection level of about 0.04 pmol per sample by using standards prepared by illumination of spectrophotometrically determined amounts of rhodopsin. The antiserum shows considerable cross-reactivity with opsin from several species (mouse, rat, quail, monkey and man). The high degree of monospecificity and cross-reactivity of the antiserum already allowed quantitation of opsin content in crude eye extracts of mouse, rat and quail with a sensitivity comparable to that of bovine opsin. Similar types of multispecies immunoassays for quantitation of highly conserved membrane proteins can be developed using the described approach, requiring only a monospecific antiserum elicited against an easily accessible species and crude tissue extracts both for coating and as a source of the inhibi...Continue Reading

References

Feb 15, 1983·Biochimica Et Biophysica Acta·J J SchalkenF J Daemen
Aug 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J Nathans, D S Hogness
Jan 1, 1983·Vision Research·R J PetersonR W Jackson
Jan 1, 1980·Journal of Immunoassay·A H Schuurs, B K van Weemen
Dec 1, 1982·Experimental Eye Research·J J PlantnerE L Kean
Jan 1, 1980·Methods in Enzymology·W J De GripS L Bonting

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Citations

Jan 1, 1988·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·J J SchalkenR M Broekhuyse
Jan 1, 1995·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·H P HofsW J De Grip
May 29, 2003·Journal of Insect Physiology·N GaoJ Hardie
Dec 1, 1988·Photochemistry and Photobiology·W J DeGrip
Jan 1, 2014·The Journal of Comparative Neurology·Tomoki IsayamaClint L Makino
Feb 27, 2003·The European Journal of Neuroscience·Rafael Cernuda-CernudaWillem J DeGrip
Aug 8, 2013·Protein Expression and Purification·Nazhat Shirzad-WaseiWillem J Degrip
Aug 1, 1989·Journal of Comparative Physiology. A, Sensory, Neural, and Behavioral Physiology·R G FosterW J De Grip

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