Enzyme-linked immunosorbent assay for amitriptyline and other antidepressants using a monoclonal antibody

Clinica Chimica Acta; International Journal of Clinical Chemistry
H DenisA D Strosberg

Abstract

We describe and evaluate a method to measure amitriptyline and other tricyclic antidepressants by enzyme linked immunosorbent assay, using monoclonal antibody. In this assay, biological samples were first incubated with the antibody; in a second step, free remaining antibody was allowed to bind to lysozyme-nortriptyline coated immunotitration plates. The bound fraction of the monoclonal antibody was revealed with rabbit anti-mouse serum coupled to horseradish peroxidase. The optical density of the reaction product was measured with a colorimeter at 410 nm. Specificity of the antibody was investigated by means of a Farr test showing interferences in therapeutic ranges only for chlorpromazine and phenytoine. Means of intra- and inter-assay variations were 10 and 13%, respectively. The results when compared to those obtained by gas chromatography with a selective nitrogen detector gave a correlation coefficient of 0.897. Finally, the great reliability of the monoclonal antibody, the advantages of a decreased analysis time, low cost and high capacity of the procedure contribute to make this immunoassay most suitable for clinical monitoring and pharmacokinetic studies of tricyclic antidepressants.

References

Jun 8, 1979·JAMA : the Journal of the American Medical Association·L E Hollister
Apr 1, 1979·British Journal of Clinical Pharmacology·D J BrunswickJ Mendels
Sep 21, 1978·Journal of Chromatography·B Mellström, R Braithwaite
Jun 17, 1972·Lancet·R A BraithwaiteA Coppen
Jul 1, 1983·Human Toxicology·A H Stead, A C Moffat
Aug 1, 1976·British Journal of Clinical Pharmacology·G W AherneV Marks

❮ Previous
Next ❯

Citations

Jul 2, 1998·Journal of Pharmaceutical Sciences·M P San AndrésD Pérez-Bendito

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Antipsychotic Drugs

Antipsychotic drugs are a class of medication primarily used to manage psychosis (including delusions, hallucinations, paranoia or disordered thought), principally in schizophrenia and bipolar disorder. Discover the latest research on antipsychotic drugs here