PMID: 3758919Jul 1, 1986Paper

Enzyme-linked immunosorbent assay (micro-ELISA) for serological diagnosis of multilocular echinococcosis

[Hokkaido igaku zasshi] The Hokkaido journal of medical science
M NakaoR Doi

Abstract

Multilocular echinococcosis caused by the larval stage of Echinococcus multilocularis is an important zoonosis with high mortality. In Hokkaido, Japan, although the prevalence among inhabitants has been very low, the areas infested with the parasite are rapidly spreading in recent years. Therefore, we improved on the procedure of the enzyme-linked immunosorbent assay (micro-ELISA) for serological diagnosis of multilocular echinococcosis to carry out the epidemiological survey. A micro-ELISA plate (M129A, DYNATECH), antigen of E. multilocularis cyst extract, peroxidase-conjugated anti-human IgG (MILES-YEDA), O-phenylenediamine and a plate reader (Multiskan MC, TITERTEK) were used for the assay. Reactions were performed with 0.1 ml of reagents in wells of plate at each step and 0.1 ml of 0.2N H2SO4 was finally added for stop of the reaction. The optimum condition was determined by block titration, as 10 micrograms/ml of protein for antigen concentration, 1:40 for serum dilution and 1:4,000 for conjugate dilution. In this assay system, 30 serum samples could be examined in one plate. After correcting the absorbance of negative control to 0.300, positive range was defined as more than 0.600 which was twice the value of negative con...Continue Reading

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.