Enzyme-linked immunosorbent assay to detect Lawsonia intracellularis in rabbits with proliferative enteropathy

The Journal of Veterinary Medical Science
Masahisa WataraiHidefumi Furuoka

Abstract

Lawsonia intracellularis is an obligate intracellular pathogenic bacterium that causes proliferative enteropathy in domestic and experimental animals. Antiserum against synthetic peptides of the Lawsonia surface antigen (LsaA) well recognized L. intracellularis in infected ileum by immunohistochemistry. The synthetic peptides in LsaA showed strong reaction with serum from rabbits infected with L. intracellularis by enzyme-linked immunosorbent assay. These results suggest that ELISA used synthetic peptides in LsaA and anti-LsaA serum might be useful to diagnose for proliferative enteropathy.

References

May 1, 1993·Journal of Clinical Microbiology·G H LawsonR A Mackie
Apr 1, 1996·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·N M WilliamsC J Gebhart
Aug 17, 2001·Veterinary Microbiology·D G Smith, G H Lawson
Sep 26, 2002·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Roberto M C GuedesRebecca A Mackie-Nuss
Nov 9, 2002·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Roberto M C GuedesNathan L Winkelman

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Citations

Apr 2, 2011·Acta Veterinaria Scandinavica·Magdalena JacobsonUlf Emanuelson
Apr 6, 2006·Animal Health Research Reviews·Jeremy J KrollD L Hank Harris
Oct 4, 2012·The Veterinary Journal·Joanne L Platell, Justine S Gibson
May 7, 2005·The Journal of Veterinary Medical Science·Masahisa WataraiHidefumi Furuoka
Aug 8, 2008·The Journal of Veterinary Medical Science·Masahisa WataraiHidefumi Furuoka

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