Enzyme-linked immunosorbent assay with purified Trypanosoma cruzi excreted superoxide dismutase

Clinical Biochemistry
Héctor MateoClotilde Marín

Abstract

Purification and biochemical characterization of a highly immunogenic extracellular superoxide dismutase of Trypanosoma cruzi Maracay strain. Fractionation with ammonium sulphate (35-85%) and two continuous chromatography processes (ion exchange and filtration). The molecular mass of the excreted SOD purified was 25 kDa and the isoelectric point 3.9. This enzyme was validated by ELISA with the polyclonal sera obtained from Balb-C mice and with sera from 222 chagasic patients from Brazil, Chile, Colombia, Mexico and Peru plus 20 sera corresponding to leishmaniasis from Peru. All the sera from Brazil stored since 1980 were positive, 93% were positive in chronic patients with 10 years of Chagas treatment from Chile, and 87% of the patients with pace-makers in a chronic phase from Colombia. In comparison with the IFA test, the ELISA-SODe-CRU yield was 85.59% sensitivity and specificity 100%. This method may be helpful for the diagnosis of Chagas disease.

References

Oct 15, 1977·Experientia·A Boveris, A O Stoppani
Jan 1, 1991·Experimental Parasitology·M F GonçalvesW Colli
Jul 1, 1991·Experimental Parasitology·M C BonaldoS Goldenberg
Nov 1, 1989·The American Journal of Tropical Medicine and Hygiene·M J LevinM B Rosenbaum
May 15, 1989·Molecular and Biochemical Parasitology·J L AffranchinoA C Frasch
Jan 1, 1988·Free Radical Biology & Medicine·J M McCord, I Fridovich
Jan 1, 1987·CRC Critical Reviews in Biochemistry·J V BannisterG Rotilio
Feb 1, 1996·Molecular and Biochemical Parasitology·P BécuweD Dive
Nov 1, 1996·Transfusion·N A SallesA Saéz-Alquézar
Jun 1, 1997·Molecular and Biochemical Parasitology·S O IsmailL Gedamu
Mar 21, 1998·The American Journal of Tropical Medicine and Hygiene·G M KrautzD M Engman
Aug 15, 1998·Journal of Clinical Microbiology·W M OelemannJ M Peralta
Nov 14, 1998·Current Opinion in Hematology·S Wendel
Mar 11, 1999·The American Journal of Tropical Medicine and Hygiene·M L GomesE Chiari
Apr 16, 1999·Journal of Clinical Microbiology·E S UmezawaJ F da Silveira
May 30, 2001·Trends in Parasitology·J F da SilveiraA O Luquetti
Aug 31, 2001·Clinical and Diagnostic Laboratory Immunology·M NakazawaY M Gomes
Mar 2, 2002·The Lancet Infectious Diseases·A Prata
Jun 8, 2002·Diagnostic Microbiology and Infectious Disease·Gláucia Elisete Barbosa MarconSandra Cecília Botelho Costa
May 29, 2003·FEMS Immunology and Medical Microbiology·Senobia TellesDiana A Henriquez
Feb 4, 2006·Journal of Clinical Microbiology·Mariolga BerrizbeitiaBrian J Ward
Oct 31, 2006·Memórias do Instituto Oswaldo Cruz·Clotilde MarínManuel Sánchez-Moreno
Jul 2, 2008·Memórias do Instituto Oswaldo Cruz·Héctor MateoManuel Sánchez-Moreno

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Citations

Jan 28, 2014·Transactions of the Royal Society of Tropical Medicine and Hygiene·Clotilde MarínManuel Sánchez-Moreno
Feb 5, 2013·Diagnostic Microbiology and Infectious Disease·Pasquale PierimarchiAnnalucia Serafino
Jun 19, 2013·Comparative Immunology, Microbiology and Infectious Diseases·Silvia S LongoniClotilde Marín
Feb 23, 2019·The Journal of Experimental Medicine·Lucía PiacenzaRafael Radi

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